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|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Hamster|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA1-40281 detects membrane and receptor bound TNF alpha from mouse and rat samples.
PA1-40281 has been successfully used in flow cytometry, ELISA, functional assayas, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot applications. This antibody shows no neutralizing activity.
The PA1-40281 imunogen is mouse TNF-alpha.
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effect of topical chamomile on immunohistochemical levels of IL-1β and TNF-α in 5-fluorouracil-induced oral mucositis in hamsters.
PA1-40281 was used in immunohistochemistry to study the immunohistochemical expression of IL-1beta and TNFalpha in a hamster model of oral mucositis and the effect of chamomile treatment
|Curra M,Martins MA,Lauxen IS,Pellicioli AC,Sant'Ana Filho M,Pavesi VC,Carrard VC,Martins MD||Cancer chemotherapy and pharmacology (71:293)||2013|
cachectin; TNFA; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
DIF; RATTNF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2