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Invitrogen
TNF alpha Polyclonal Antibody
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FIGURE: 1 / 13
TNF alpha Antibody (PA1-40281) in ICC/IF
Immunofluorescence analysis of TNF-Alpha (Tumor necrosis factor-alpha) was performed using 70% confluent log phase RAW 264.7 treated with lipopolysaccharide (LPS) (10 ng/mL for 7 hr) and protein transport inhibitor cocktail [500X] (PTI) [1X for 4 hr]. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at ... View More
Product Details
PA1-40281
Applications
Tested Dilution
Publications
Product Specifications
Published species
Hamster,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse TNF-alpha.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-40281 detects membrane and receptor bound TNF alpha from mouse and rat samples.
PA1-40281 has been successfully used in flow cytometry, ELISA, functional assayas, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot applications. This antibody shows no neutralizing activity.
The PA1-40281 imunogen is mouse TNF-alpha.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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