|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:50|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Hamster|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||PBS with 0.1% BSA|
|Storage conditions||4° C|
PA1-40281 detects membrane and receptor bound TNF alpha from mouse and rat samples.
PA1-40281 has been successfully used in flow cytometry, ELISA, functional assayas, immunohistochemistry (frozen), immunohistochemistry (paraffin), and Western blot applications. This antibody shows no neutralizing activity.
The PA1-40281 imunogen is mouse TNF-alpha.
Tumor Necrosis Factor-Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor-bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cachectin; TNF-a; TNF-alpha; TNFA; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene Aliases: DIF; RATTNF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2
UniProt ID: (Mouse) P06804
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