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Western blot analysis of HeLa Whole Cell Lysate using PA5-19810, TNF alpha primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19810 staining of Mouse Liver Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TNF alpha.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
This antibody is predicted to react with rat, cat, chimpanzee and baboon based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Fracture induces keratinocyte activation, proliferation, and expression of pro-nociceptive inflammatory mediators.
PA5-19810 was used in immunohistochemistry to study the cellular and protein expression changes induced by fracture
|Li WW,Guo TZ,Li XQ,Kingery WS,Clark JD||Pain (151:843)||2010|
APC1 protein; cachectin; DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
DIF; RATTNF; TNF; TNF-a; TNF-alpha; TNFA; TNFalpha; Tnfsf1a; TNFSF2; TNLG1F