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Invitrogen
TNF alpha Polyclonal Antibody, Biotin
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TNF alpha Antibody (PBOTNFABI) in ELISA
ELISA analysis of TNF-alpha was performed by coating 100 µL per well of anti-bovine TNF-alpha polyclonal antibody (Product # PBOTNFAI) at a concentration of 10 µg/mL and incubating overnight at 4C. The plate was blocked for one hour using 3% bovine serum albumin (BSA) diluted in PBST. The plate was washed six times with 200 µL/well PBST, and then incubated with 100 µL per well of recombinant bovine TNF-alpha protein standards and recombinant ovine TNF-alpha in tissue culture supernatant (both Log2 dilutions starting at an unknown concentra... View More
Product Details
PBOTNFABI
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Sheep
Published species
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
TNF alpha
Conjugate
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
proprietary preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PBOTNFABI targets TNF Alpha in ELISA, and WB applications and shows reactivity with bovine and ovine samples.
The PBOTNFABI immunogen is tNF alpha.
PBOTNFABI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
The PBOTNFABI polyclonal biotinylated bovine TNFa antibody has successfully been paired as the detection antibody in a sandwich ELISA with monoclonal capture antibody MBOTNFAI (clone E10011). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2
Gene Aliases: TNF; TNF-a; TNF-alpha; TNFA; TNFSF2
UniProt ID: (Bovine) Q06599, (Sheep) P23383
Entrez Gene ID: (Bovine) 280943, (Sheep) 443540
positive regulation of protein phosphorylation
production of molecular mediator involved in inflammatory response
immune response
vascular endothelial growth factor production
positive regulation of plasminogen activation
positive regulation of apoptotic process
negative regulation of protein complex disassembly
positive regulation of protein complex disassembly
positive regulation of MAP kinase activity
positive regulation of JUN kinase activity
positive regulation of inflammatory response
positive regulation of NF-kappaB transcription factor activity
positive regulation of triglyceride metabolic process
necroptotic signaling pathway
negative regulation of lactation
negative regulation of feeding behavior
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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