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Invitrogen
TNF beta Polyclonal Antibody
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FIGURE: 1 / 7
TNF beta Antibody (PA5-116055) in ICC/IF
Immunocytochemistry analysis of TNF beta in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116055) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116055
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LTA(Accession P01374), corresponding to amino acid residues A48-S98.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TNF beta.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DAMA-25N12.13; hypothetical protein; lymphotoxin alpha isoform b; hypothetical protein; lymphotoxin alpha isoform c; hypothetical protein; lymphotoxin alpha isoform d; hypothetical protein; lymphotoxin alpha isoform e; hypothetical protein; lymphotoxin alpha isoform f; hypothetical protein; lymphotoxin alpha isoform g; LT-alpha; LTA; lymphotoxin A; lymphotoxin alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin-alpha; TNF b; TNF beta; TNF superfamily, member 1; TNF β; TNF-beta; TNFbeta; TNFβ; tumor necrosis factor beta; tumor necrosis factor ligand 1E; Tumor necrosis factor ligand superfamily member 1; unnamed protein product
Gene Aliases: hlb382; LT; LT-[a]; LT-alpha; LT[a]; LTA; LTalpha; Ltx; TNF-beta; TNFB; TNFSF1; Tnfsf1b; TNLG1E
UniProt ID: (Human) P01374, (Mouse) P09225, (Rat) Q06332
Entrez Gene ID: (Human) 4049, (Mouse) 16992, (Rat) 25008
response to hypoxia
positive regulation of chronic inflammatory response to antigenic stimulus
positive regulation of humoral immune response mediated by circulating immunoglobulin
apoptotic process
immune response
humoral immune response
cell communication
signal transduction
cell surface receptor signaling pathway
cell-cell signaling
response to nutrient
response to xenobiotic stimulus
signaling
response to lipopolysaccharide
positive regulation of interferon-gamma production
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of fibroblast proliferation
lymph node development
defense response to Gram-positive bacterium
positive regulation of glial cell proliferation
positive regulation of extrinsic apoptotic signaling pathway
obsolete transformed cell apoptotic process
inflammatory response
cell proliferation
positive regulation of cell proliferation
cellular process
negative regulation of growth of symbiont in host
response to drug
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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