Invitrogen

TOMM20 Polyclonal Antibody

TOMM20 Antibody in Immunocytochemistry (ICC/IF)

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TOMM20 Antibody (PA5-52843) in ICC/IF

Immunofluorescence analysis of TOMM20 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TOMM20 Polyclonal Antibody (Product # PA5-52843) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), ... View More

TOMM20 Antibody in Immunohistochemistry (IHC)

TOMM20 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

PA5-52843

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Horse, Human, Mouse, Rat, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TOMM20. Recombinant protein control fragment (Product #RP-90451).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2648808

Product Specific Information

Immunogen sequence: KRRSDPNFKN RLRERRKKQK LAKERAGLSK LPDLKDAEAV QKFFLEEIQL GEELLAQGEY EKGVDHLTNA IAVCGQPQQL LQVLQQTLPP PVFQMLLTKL PTISQRIVSA QSLAEDDV

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.

Target Information

The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Western Blot

Sat Aug 19 00:00:00 UTC 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Western Blot

Sat Aug 19 00:00:00 UTC 2023

A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes.

Animal models and experimental medicine

Sheng H,Lu D,Qi X,Ling Y,Li J,Zhang X,Dong W,Chen W,Gao S,Gao X,Zhang L,Zhang L

PA5-52843 was used in Western Blotting to establish a rat model simulating MMDS5 disease in the nervous system to investigate its pathological features and neuronal death.

Sat Apr 01 00:00:00 UTC 2023

Nitration-mediated activation of the small GTPase RhoA stimulates cellular glycolysis through enhanced mitochondrial fission.

The Journal of biological chemistry

Lu Q,Sun X,Yegambaram M,Ornatowski W,Wu X,Wang H,Garcia-Flores A,Da Silva V,Zemskov EA,Tang H,Fineman JR,Tieu K,Wang T,Black SM

PA5-52843 was used in Immunocytochemistry to identify a novel link between RhoA, mitochondrial fission, and cellular glycolysis and suggest that targeting RhoA nitration could have therapeutic benefits for treating PH.

Sat Apr 01 00:00:00 UTC 2023

Isolation of Extracellular Vesicles From the Bronchoalveolar Lavage Fluid of Healthy and Asthmatic Horses.

Frontiers in veterinary science

Höglund N,Koho N,Rossi H,Karttunen J,Mustonen AM,Nieminen P,Rilla K,Oikari S,Mykkänen A

PA5-52843 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate the number and size distribution of EVs in the BALF of asthmatic horses (EA, n = 35) and healthy horses (n = 19).

Sat Jul 16 00:00:00 UTC 2022

The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons.

Stem cell reports

Poon A,Saini H,Sethi S,O'Sullivan GA,Plun-Favreau H,Wray S,Dawson LA,McCarthy JM

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Western Blot

Tue May 11 00:00:00 UTC 2021

Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathy.

Animal models and experimental medicine

Ling Y,Ma J,Qi X,Zhang X,Kong Q,Guan F,Dong W,Chen W,Gao S,Gao X,Pan S,Ma Y,Lu D,Zhang L

PA5-52843 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to establish a rat model of MMDS complicated with cardiomyopathy which can be applied to the study of the mechanism of energy metabolism in cardiovascular diseases, as well as research and development of drugs.

Wed Dec 01 00:00:00 UTC 2021

Nix-Mediated Mitophagy Modulates Mitochondrial Damage During Intestinal Inflammation.

Antioxidants & redox signaling

Vincent G,Novak EA,Siow VS,Cunningham KE,Griffith BD,Comerford TE,Mentrup HL,Stolz DB,Loughran P,Ranganathan S,Mollen KP

PA5-52843 was used in Immunohistochemistry to demonstrate the importance of mitophagy within the intestinal epithelium during IBD pathogenesis.

Wed Jul 01 00:00:00 UTC 2020

Hypoxic Regulation of Mitochondrial Metabolism and Mitophagy in Nucleus Pulposus Cells Is Dependent on HIF-1α-BNIP3 Axis.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Madhu V,Boneski PK,Silagi E,Qiu Y,Kurland I,Guntur AR,Shapiro IM,Risbud MV

PA5-52843 was used in Western Blot to provide novel mechanistic insights into the complex interplay between hypoxia and HIF-1α signaling on the mitochondrial metabolism and quality control in NP cells.

Sat Aug 01 00:00:00 UTC 2020

Fitter Mitochondria Are Associated With Radioresistance in Human Head and Neck SQD9 Cancer Cells.

Frontiers in pharmacology

Grasso D,Medeiros HCD,Zampieri LX,Bol V,Danhier P,van Gisbergen MW,Bouzin C,Brusa D,Grégoire V,Smeets H,Stassen APM,Dubois LJ,Lambin P,Dutreix M,Sonveaux P

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Immunocytochemistry

Mon Sep 28 00:00:00 UTC 2020

Fitter Mitochondria Are Associated With Radioresistance in Human Head and Neck SQD9 Cancer Cells.

Frontiers in pharmacology

Grasso D,Medeiros HCD,Zampieri LX,Bol V,Danhier P,van Gisbergen MW,Bouzin C,Brusa D,Grégoire V,Smeets H,Stassen APM,Dubois LJ,Lambin P,Dutreix M,Sonveaux P

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Immunocytochemistry

Mon Sep 28 00:00:00 UTC 2020

Changes of Metabolic Phenotype of Cardiac Progenitor Cells During Differentiation: Neutral Effect of Stimulation of AMP-Activated Protein Kinase.

Stem cells and development

André E,De Pauw A,Verdoy R,Brusa D,Bouzin C,Timmermans A,Bertrand L,Balligand JL

PA5-52843 was used in Immunocytochemistry to investigate the pharmacological modulation involved in the metabolic shift of cardiac progenitor cells.

Fri Nov 15 00:00:00 UTC 2019

Changes of Metabolic Phenotype of Cardiac Progenitor Cells During Differentiation: Neutral Effect of Stimulation of AMP-Activated Protein Kinase.

Stem cells and development

André E,De Pauw A,Verdoy R,Brusa D,Bouzin C,Timmermans A,Bertrand L,Balligand JL

PA5-52843 was used in Immunocytochemistry to investigate the pharmacological modulation involved in the metabolic shift of cardiac progenitor cells.

Fri Nov 15 00:00:00 UTC 2019

APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress.

Cell death & disease

Pan JX,Tang F,Xiong F,Xiong L,Zeng P,Wang B,Zhao K,Guo H,Shun C,Xia WF,Mei L,Xiong WC

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Immunofluorescence

Mon Oct 22 00:00:00 UTC 2018

APP promotes osteoblast survival and bone formation by regulating mitochondrial function and preventing oxidative stress.

Cell death & disease

Pan JX,Tang F,Xiong F,Xiong L,Zeng P,Wang B,Zhao K,Guo H,Shun C,Xia WF,Mei L,Xiong WC

Published figure using TOMM20 polyclonal antibody (Product # PA5-52843) in Immunofluorescence

Mon Oct 22 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; Outer mitochondrial membrane receptor Tom20; translocase of outer mitochondrial membrane 20 homolog type II

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Gene Aliases: 1810060K07Rik; BB284719; Gm19268; KIAA0016; MAS20; mKIAA0016; MOM19; TOM20; TOMM20

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UniProt ID: (Human) Q15388, (Mouse) Q9DCC8

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Entrez Gene ID: (Human) 9804, (Mouse) 67952

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Function(s)

protein binding protein channel activity P-P-bond-hydrolysis-driven protein transmembrane transporter activity mitochondrion targeting sequence binding unfolded protein binding primary active transporter

Process(es)

protein targeting to mitochondrion tRNA import into mitochondrion macroautophagy protein import into mitochondrial matrix mitochondrial outer membrane translocase complex assembly protein targeting transport intracellular protein transport protein transport protein transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TOMM20 Polyclonal Antibody

TOMM20 Antibody (PA5-52843) in ICC/IF

Product Details

PA5-52843

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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