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Invitrogen
TOMM20 Polyclonal Antibody
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FIGURE: 1 / 14
TOMM20 Antibody (PA5-78300) in ICC/IF
Immunofluorescence analysis was performed using Anti-TOMM20 Rabbit Polyclonal Antibody (Product # PA5-78300) using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOMM20 Rabbit Monoclonal Antibody (Product # PA5-78300) at 1:250 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Supe... View More
Product Details
PA5-78300
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length Human TOMM20 recombinant protein.
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; outer mitochondrial membrane receptor rTOM20; Outer mitochondrial membrane receptor Tom20; translocase of outer mitochondrial membrane 20 homolog type II
Gene Aliases: 1810060K07Rik; BB284719; Gm19268; KIAA0016; MAS20; mKIAA0016; MOM19; TOM20; TOMM20
UniProt ID: (Human) Q15388, (Rat) Q62760, (Mouse) Q9DCC8
Entrez Gene ID: (Human) 9804, (Rat) 266601, (Mouse) 67952
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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