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Invitrogen
TOP1 Polyclonal Antibody
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FIGURE: 1 / 8
TOP1 Antibody (PA5-78203) in ICC/IF
Immunofluorescence analysis of TOP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOP1 Rabbit Polyclonal Antibody (Product # PA5-78203) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A2703... View More
Product Details
PA5-78203
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Topoisomerase I. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, 293T NE
Predicted Reactivity: Rat (100%), Rhesus Monkey (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 1; DNA topoisomerase I; RP3-511B24.2; Topoisomerase 1; type I DNA topoisomerase
Gene Aliases: Ab2-086; TOP1; TOPI
UniProt ID: (Human) P11387, (Rat) Q9WUL0
Entrez Gene ID: (Human) 7150, (Rat) 64550
DNA replication
DNA topological change
chromatin remodeling
chromosome segregation
circadian rhythm
programmed cell death
viral process
phosphorylation
protein sumoylation
circadian regulation of gene expression
embryonic cleavage
response to drug
response to temperature stimulus
rRNA transcription
response to gamma radiation
organ regeneration
response to cAMP
cellular response to luteinizing hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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