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TORC1 Antibody (A300-768A) in IP
Detection of human TORC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TORC1 antibody BL3431 (Cat. No. A300-768A) used for IP at 3 µg/mg lysate. TORC1 was also immunoprecipitated by rabbit anti-TORC1 antibodies BL3433 and BL3434 (Cat. No. A300-769A). For blotting immunoprecipitated TORC1, BL3434 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-768A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 25 and 75 of Human Transducer of Regulated cAMP Response element-binding protein 1 (CREB Regulated Transcription Coactivator 1).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons. In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CREB-regulated transcription coactivator 1; CRTC1; FLJ14027; MECT 1; Mucoepidermoid carcinoma translocated protein 1; TORC-1; transducer of regulated cAMP response element-binding protein (CREB) 1; Transducer of regulated cAMP response element-binding protein 1
Gene Aliases: CRTC1; KIAA0616; MECT1; TORC-1; TORC1; WAMTP1
UniProt ID: (Human) Q6UUV9
Entrez Gene ID: (Human) 23373
transcription, DNA-templated
memory
viral process
positive regulation of CREB transcription factor activity
entrainment of circadian clock by photoperiod
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
protein homotetramerization
membrane hyperpolarization
positive regulation of dendrite development
positive regulation of long-term synaptic potentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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