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Bethyl Laboratories
TORC2 Polyclonal Antibody
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FIGURE: 1 / 1
TORC2 Antibody (A300-637A) in IP
Detection of human TORC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TORC2 antibody (Product # A300-637A) (lot 2) used for IP at 6 µg per reaction. TORC2 was also immunoprecipitated by a previous lot of this antibody (Product # A300-637A) (lot 1) and another antibody against a different epitope of TORC2 (A300-638A). For blotting immunoprecipitated TORC2, A300-638A was used at 0.4 µg... View More
Product Details
A300-637A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 300 and 350 of Human Transducer of Regulated cAMP Responsive Element-Binding Protein (CREB) 2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat
Target Information
CRTC2, also known as TORC2 (Transducers Of Regulated cAMP Response Element-Binding (CREB)) 2 and the related protein CRTC1 are potent CREB coactivators that are exported from the nucleus in a CRM1-dependent manner via phosphorylation-dependent interactions. Studies suggest that their phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP. CRTC2 is present in both B and T-lymphocytes and abundantly expressed in the thymus. Its activity is important in regulating the expression of genes involved in cellular energy metabolism while CRTC1 is essential for energy balance and fertility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CREB-regulated transcription coactivator 2; OTTHUMP00000035255; TORC-2; transcriptional co-activator; transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; unnamed protein product
Gene Aliases: CRTC2; TORC-2; TORC2
UniProt ID: (Human) Q53ET0
Entrez Gene ID: (Human) 200186
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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