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Bioss
TPA Polyclonal Antibody
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FIGURE: 1 / 1
TPA Antibody (BS-17162R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TPA Polyclonal Antibody, Unconjugated (bs-17162R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-17162R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human Tissue-type plasminogen activator chain B, amino acids 401-500.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.4.21.68; alteplase; plasminogen/activator kringle; reteplase; t plasminogen activator; t-PA; t-plasminogen activator; tissue plasminogen activator; Tissue-type plasminogen activator
Gene Aliases: AU020998; AW212668; D8Ertd2e; PATISS; PLAT; T-PA; TPA
UniProt ID: (Human) P00750, (Mouse) P11214, (Rat) P19637
Entrez Gene ID: (Human) 5327, (Mouse) 18791, (Rat) 25692
response to hypoxia
cellular protein modification process
proteolysis
blood coagulation
smooth muscle cell migration
plasminogen activation
fibrinolysis
negative regulation of proteolysis
platelet-derived growth factor receptor signaling pathway
synaptic transmission, glutamatergic
positive regulation of ovulation
wound healing
response to peptide hormone
regulation of synaptic plasticity
response to cAMP
cellular response to dexamethasone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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