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TPC2 Polyclonal Antibody
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FIGURE: 1 / 10
TPC2 Antibody (OST00270G-500UG) in IHC (P)
IHC-P on paraffin sections of human gallbladder. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 10 µg/mL, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Product Details
OST00270G-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 700-752 of human TPC2 conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity of this antibody: TPC2.
Target Information
Tpcn2 is a nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. Tpcn2 may also be involved in smooth muscle contraction. The Tpcn2 gene encodes a putative cation-selective ion channel with two repeats of a six-transmembrane-domain. The Tpcn2 protein localizes to lysosomal membranes and enables nicotinic acid adenine dinucleotide phosphate (NAADP) -induced calcium ion release from lysosome-related stores. Tpcn2 is a ubiquitously expressed gene that has elevated expression in liver and kidney. Two common nonsynonymous SNPs in the Tpcn2 gene strongly associate with blond versus brown hair pigmentation. Diseases associated with TPCN2 include Deafness, Autosomal Recessive 63 and Deafness, Autosomal Recessive 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Two pore calcium channel protein 2; Two pore channel protein 2; two-pore calcium channel protein 2; voltage-dependent calcium channel protein TPC2
Gene Aliases: SHEP10; TPC2; TPCN2
UniProt ID: (Human) Q8NHX9
Entrez Gene ID: (Human) 219931
cellular calcium ion homeostasis
smooth muscle contraction
lysosome organization
regulation of autophagy
calcium-mediated signaling
response to vitamin D
ion transmembrane transport
regulation of ion transmembrane transport
release of sequestered calcium ion into cytosol
membrane depolarization during action potential
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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