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Invitrogen
TPH1 Polyclonal Antibody
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FIGURE: 1 / 8
TPH1 Antibody (PA1-777) in ICC/IF
Immunofluorescent analysis of TPH1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TPH1 polyclonal antibody (Product # PA1-777) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugat... View More
Product Details
PA1-777
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rabbit,
Rat,
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C-terminal region of TPH1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-777 detects TPH1 in human, mouse, rat, rabbit and non-human primate samples.
PA1-777 has been successfully used in ICC/IF, IP, and Western blot procedures. By Western blot PA1-777 detects a 51 kDa protein representing TPH1.
The PA1-777 immunogen is a synthetic peptide corresponding to the C-terminal region of TPH1.
Target Information
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1 (tryptophan 5-monooxygenase); unnamed protein product
Gene Aliases: TPH; TPH1; TPH2; TPRH; TRPH
UniProt ID: (Human) P17752, (Rabbit) P17290, (Mouse) P17532, (Rat) P09810
Entrez Gene ID: (Human) 7166, (Rabbit) 100101558, (Rabbit) 100009100, (Mouse) 21990, (Rat) 24848
monooxygenase activity
tryptophan 5-monooxygenase activity
iron ion binding
protein binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
metal ion binding
amino acid binding
platelet degranulation
serotonin biosynthetic process from tryptophan
aromatic amino acid family metabolic process
serotonin biosynthetic process
positive regulation of fat cell differentiation
bone remodeling
mammary gland alveolus development
regulation of hemostasis
metabolic process
negative regulation of ossification
oxidation-reduction process
circadian rhythm
response to immobilization stress
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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