Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (96%), Rat (96%), Japanese Medaka (89%), Xenopus laevis (90%), Dog (99%), Rabbit (98%), Chicken (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; Neuronal tryptophan hydroxylase; TPRH; TRPH; Tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 1; Tryptophan 5-monooxygenase 2; tryptophan hydroxylase (tryptophan 5-monooxygenase)
Gene Aliases: ADHD7; NTPH; TPH; TPH1; TPH2; TPRH; TRPH