Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (PA1-778). PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)|
|Storage buffer||1mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.
PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.
The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis.
Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Associations among within-litter differences in early mothering received and later emotional behaviors, mothering, and cortical tryptophan hydroxylase-2 expression in female laboratory rats.
PA1-778 was used in western blot to study how variability in maternal care received among female rat siblings affects the siblings' later emotional and maternal behaviors
|Ragan CM,Harding KM,Lonstein JS||Hormones and behavior (77:62)||2016|
Sympathetic regulation of vascular tone via noradrenaline and serotonin in the rat carotid body as revealed by intracellular calcium imaging.
PA1-778 was used in immunohistochemistry - frozen section to study hypoxia-induced chemosensory activity in the carotid body.
|Yokoyama T,Nakamuta N,Kusakabe T,Yamamoto Y||Brain research (1596:126)||2015|
Evaluation of blood-brain barrier permeability in tryptophan hydroxylase 2-knockout mice.
PA1-778 was used in immunohistochemistry to demonstrate that TPH2-knockout mice have a normal blood brain barrier
|Xu CJ,Dai TL,Niu XY,Wang JL,Jin MS||Experimental and therapeutic medicine (8:1467)||2014|
Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.
PA1-778 was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats
|Castro B,Sánchez P,Torres JM,Ortega E||PloS one (8:null)||2013|
Differential tissue distribution of tryptophan hydroxylase isoforms 1 and 2 as revealed with monospecific antibodies.
PA1-778 was used in western blot to investigate the localization of TPH isoforms in various tissues
|Sakowski SA,Geddes TJ,Thomas DM,Levi E,Hatfield JS,Kuhn DM||Brain research (1085:11)||2006|