|Western Blot (WB)||1:1,000|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)|
|Storage buffer||1mg/ml BSA|
|Contains||0.1% sodium azide|
|Storage conditions||4° C|
PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.
PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.
The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis.
Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Neuronal tryptophan hydroxylase; NTPH; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2; tryptophan hydroxylase 2
Gene Aliases: ADHD7; AU043594; NTPH; TPH2
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