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Invitrogen
TPH2 Polyclonal Antibody
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FIGURE: 1 / 14
TPH2 Antibody (PA1-778) in IP
Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (Product # PA1-778). Product # PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody ... View More
Product Details
PA1-778
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.
PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.
The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.
Target Information
Tryptophan hydroxylase (TPH) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC119994; Neuronal tryptophan hydroxylase; TPRH; TRPH; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2
Gene Aliases: ADHD7; AU043594; NTPH; TPH2
UniProt ID: (Human) Q8IWU9, (Mouse) Q8CGV2, (Rat) Q8CGU9
Entrez Gene ID: (Human) 121278, (Rabbit) 100344397, (Mouse) 216343, (Rat) 317675
tryptophan 5-monooxygenase activity
iron ion binding
amino acid binding
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
metal ion binding
molecular_function
circadian rhythm
aromatic amino acid family metabolic process
response to activity
response to nutrient levels
serotonin biosynthetic process
response to estrogen
indolalkylamine biosynthetic process
response to glucocorticoid
response to calcium ion
oxidation-reduction process
cellular response to lithium ion
serotonin biosynthetic process from tryptophan
metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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