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TPI1 Antibody (PA5-18342) in ICC/IF
Immunofluorescence analysis of TPI1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TPI1 Polyclonal Antibody (Product # PA5-18342) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution... View More
Product Details
PA5-18342
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (LKPEFVDIINAKQ) corresponding to the C-terminus amino acids of TPI1 (aa 237-249).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10981991
Product Specific Information
This antibody is predicted to react with bovine, canine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
Target Information
This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate and dihydroxy-acetone phosphate in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 49; Methylglyoxal synthase; MGC88108; TIM; Triose-phosphate isomerase; Triosephosphate isomerase
Gene Aliases: AI255506; HEL-S-49; TIM; TPI; Tpi-1; TPI1; TPID
UniProt ID: (Human) P60174, (Dog) P54714, (Rat) P48500, (Mouse) P17751
Entrez Gene ID: (Human) 7167, (Dog) 477711, (Rat) 24849, (Mouse) 21991
gluconeogenesis
glycolytic process
pentose-phosphate shunt
multicellular organism development
glycerol catabolic process
glyceraldehyde-3-phosphate biosynthetic process
canonical glycolysis
methylglyoxal biosynthetic process
carbohydrate metabolic process
glucose metabolic process
metabolic process
embryo development
glyceraldehyde-3-phosphate metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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