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Invitrogen
TPL2 Polyclonal Antibody
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FIGURE: 1 / 7
TPL2 Antibody (PA5-115541) in IHC (P)
Immunohistochemistry analysis of TPL2 in human brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with TPL2 polyclonal antibody (Product # PA5-115541) using a dilution of 1:100 (1.5 hours at 22°C) followed by HRP conjugated goat anti-rabbit secondary antibody.
Product Details
PA5-115541
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MAP3K8(Accession P41279), corresponding to amino acid residues T264-G314.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MAP3K8/COT.
Target Information
MAP3K8 (COT) is a serine/threonine kinase which can activate both the MAP and JNK kinase pathways and plays a role in the cell cycle. Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: augmented in rheumatoid arthritis 2; Cancer Osaka thyroid oncogene; cancer Osaka thyroid, oncogene; CCOT; cot (cancer Osaka thyroid) oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 1.14.16.2; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K 8; MAP3K8; mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Protein kinase cot; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; serine/threonine kinase (Tpl-2); Serine/threonine-protein kinase cot; TH isoform 3; TH isoform a; TH-4; TPL 2; TPL-2; Tumor progression locus 2; TY3H; TYH; unnamed protein product
Gene Aliases: AURA2; c-COT; COT; Cot/Tpl2; D17TPL2; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
UniProt ID: (Human) P41279, (Mouse) Q07174, (Rat) Q63562
Entrez Gene ID: (Human) 1326, (Mouse) 26410, (Rat) 116596
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
receptor signaling protein serine/threonine kinase activity
non-receptor serine/threonine protein kinase
MAPK cascade
immune system process
protein phosphorylation
signal transduction
T cell costimulation
positive regulation of inflammatory response
cell cycle
regulation of mitotic cell cycle
phosphorylation
signal transduction by protein phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
activation of MAPKK activity
apoptotic process
intracellular signal transduction
regulation of cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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