Invitrogen

TPL2 Recombinant Polyclonal Antibody (3HCLC)

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TPL2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of COT/TPL2/MAP3K8 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COT/TPL2/MAP3K8 (3HCLC), Recombinant Rabbit Polyclonal Antibody (Product # 710377) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

TPL2 Antibody in Western Blot (WB) — Western blot analysis of TPL2 in whole cell extracts from Jurkat cells (lane 1), 3T3L1 cells (lane 2), and HepG2 cells (lane 3) using a TPL2 Recombinant Rabbit Polyclonal Antibody (Product # 710377) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~52kDa.

Product Details

710377

Applications	Tested Dilution	Publications
Western Blot (WB)	2-3 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2-3 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Recombinant Polyclonal
Type	Antibody
Clone	3HCLC
Immunogen	Peptide corresponding to amino acids 282–293 of human COT/TPL2/MAP3K8 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532699

Product Specific Information

This antibody is predicted to react with bovine, canine, equine, mouse, rabbit and rat based on sequence homology.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. The protein appears to play an important role in the cell cycle. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Janus Kinase 1 Plays a Critical Role in Mammary Cancer Progression.

Cell reports

Wehde BL,Rädler PD,Shrestha H,Johnson SJ,Triplett AA,Wagner KU

710377 was used in Western Blotting to determine the role of Janus kinases in IL-6 cytokine signalling in metastatic cancer.

Tue Nov 20 00:00:00 EST 2018

Bioinformatics

Protein Aliases: augmented in rheumatoid arthritis 2; Cancer Osaka thyroid oncogene; cancer Osaka thyroid, oncogene; CCOT; cot (cancer Osaka thyroid) oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 1.14.16.2; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K 8; mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Protein kinase cot; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TH isoform 3; TH isoform a; TH-4; TPL 2; TPL-2; Tumor progression locus 2; TY3H; TYH

Gene Aliases: AURA2; c-COT; COT; Cot/Tpl2; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2

UniProt ID: (Human) P41279, (Mouse) Q07174

Entrez Gene ID: (Human) 1326, (Mouse) 26410

Molecular Function: non-receptor serine/threonine protein kinase