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Invitrogen
TPM1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
TPM1 Antibody (PA5-29846) in ICC/IF
Immunofluorescence analysis of TPM1 was performed using 70% confluent log phase HSkMs cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TPM1 Rabbit Polyclonal Antibody (Product # PA5-29846) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA5-29846
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 92 and 273 of Human Tropomyosin 1
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547320
Product Specific Information
Recommended positive controls: A431, mouse liver, rat muscle.
Predicted reactivity: Mouse (99%), Rat (88%), Zebrafish (80%), Xenopus laevis (83%), Pig (88%), Rabbit (88%), Chicken (81%), Sheep (88%), Bovine (88%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha tropomyosin; Alpha-tropomyosin; cardiomyopathy, hypertrophic 3; epididymis secretory protein Li 265; hepatoma alpha tropomyosin; sarcomeric tropomyosin kappa; smooth muscle alpha-tropomyosin; striated muscle alpha-tropomyosin; TPM1-alpha; tropomyosin; tropomyosin 1 (alpha); tropomyosin 3 alpha; Tropomyosin alpha-1 chain; Tropomyosin-1
Gene Aliases: AA986836; AI854628; Alpha-tm; C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; TM2; Tm3; Tma2; Tmpa; TMSA; Tpm-1; TPM1; Tpma
UniProt ID: (Human) P09493, (Pig) P42639, (Rat) P04692, (Mouse) P46902
Entrez Gene ID: (Human) 7168, (Pig) 100037999, (Rat) 24851, (Mouse) 22003
actin binding
structural constituent of cytoskeleton
protein binding
cytoskeletal protein binding
structural constituent of muscle
identical protein binding
protein homodimerization activity
protein heterodimerization activity
actin filament binding
protein N-terminus binding
actin binding motor protein
in utero embryonic development
positive regulation of heart rate by epinephrine
movement of cell or subcellular component
muscle contraction
regulation of muscle contraction
cytoskeleton organization
regulation of heart contraction
regulation of cell shape
muscle filament sliding
negative regulation of cell migration
ruffle organization
positive regulation of ATPase activity
cellular response to reactive oxygen species
wound healing
sarcomere organization
positive regulation of cell adhesion
positive regulation of stress fiber assembly
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
negative regulation of vascular smooth muscle cell proliferation
negative regulation of vascular associated smooth muscle cell migration
regulation of ATPase activity
actin filament capping
embryo development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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