Bethyl Laboratories

TPR Polyclonal Antibody

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TPR Antibody in Western Blot (WB) — **Detection of human TPR by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-TPR antibody BL2149 (Cat. No. A300-826A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate (B). TPR was also immunoprecipitated by rabbit anti-TPR antibodies BL2148 (Cat. No. A300-825A), BL2150 (Cat. No. A300-827A) and BL2151 (Cat. No. A300-828A), which recognize other epitopes. *Detection:* Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).

Product Details

Tested Applications

Dilution

Immunoprecipitation (IP)

2-5 µg/mg lysate

Western Blot (WB)

1:2,000-1:10,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 1650 and 1700

Conjugate

Unconjugated

Form

liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat

Target Information

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Megator; NPC-associated intranuclear protein; nuclear basket protein; nuclear pore complex-associated protein TPR; Nucleoprotein TPR; translocated promoter region; translocated promoter region (to activated MET oncogene); Translocated promoter region protein; tumor potentiating region

Gene Aliases: TPR

UniProt ID: (Human) P12270

Entrez Gene ID: (Human) 7175

Function(s)

negative regulation of transcription from RNA polymerase II promoter MAPK import into nucleus RNA import into nucleus RNA export from nucleus mRNA export from nucleus tRNA export from nucleus protein import into nucleus protein export from nucleus nuclear pore organization mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint biological_process regulation of mRNA export from nucleus regulation of glucose transport regulation of mitotic sister chromatid separation viral process protein sumoylation viral transcription gene silencing by RNA positive regulation of heterochromatin assembly regulation of protein stability mRNA export from nucleus in response to heat stress cellular response to heat cellular response to interferon-alpha regulation of protein import into nucleus positive regulation of protein import into nucleus nuclear matrix organization negative regulation of translational initiation regulation of protein export from nucleus positive regulation of protein export from nucleus negative regulation of RNA export from nucleus nuclear pore complex assembly cell division response to epidermal growth factor intracellular transport of virus positive regulation of mitotic cell cycle spindle assembly checkpoint positive regulation of intracellular protein transport regulation of cellular response to heat regulation of mitotic spindle assembly

Process(es)

molecular_function chromatin binding mRNA binding transporter activity nucleocytoplasmic transporter activity protein binding tubulin binding heat shock protein binding protein homodimerization activity protein anchor poly(A) RNA binding mitogen-activated protein kinase binding dynein complex binding

TPR Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

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