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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TPX2 Antibodies

          Invitrogen

          TPX2 Polyclonal Antibody

          View all (15) TPX2 antibodies

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          Cite TPX2 Polyclonal Antibody

          • Antibody Testing Data (1)
          TPX2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TPX2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          TPX2 Antibody (PA5-80178) in WB

          Western blot analysis of TPX2 in Lane 1: human HeLa whole cell lysate, Lane 2: human COLO-320 whole cell lysate, Lane 3: human U-87MG whole cell lysate, Lane 4: human SGC-7901 whole cell lysate, Lane 5: rat PC-12 whole cell lysate, Lane 6: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TPX2 Antibody in Western Blot (WB)
          TPX2 Polyclonal Antibody

          Product Details

          PA5-80178

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived human TPX2 recombinant protein (Position: D15-Q93).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 4mg trehalose

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2747292

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human Hela whole cell, human COLO-320 whole cell, human U-87MG whole cell, human SGC-7901 whole cell, rat PC-12 whole cell, mouse HEPA1-6 whole cell.

          Target Information

          The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: Differentially expressed in cancerous and non-cancerous lung cells 2; differentially expressed in lung cells; DIL-2; GD: HCA519; Hepatocellular carcinoma-associated antigen 519; Hepatocellular carcinoma-associated antigen 90; p100; preferentially expressed in colorectal cancer; Protein fls353; restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX 2; TPX2, microtubule-associated protein homolog; TPX2, microtubule-associated, homolog

          View more View less

          Gene Aliases: 2610005B21Rik; C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; REPP86; TPX2

          View more View less

          UniProt ID: (Human) Q9ULW0, (Mouse) A2APB8

          View more View less

          Entrez Gene ID: (Human) 22974, (Rat) 311546, (Mouse) 72119

          View more View less

          Function(s)
          protein binding ATP binding GTP binding protein kinase binding importin-alpha family protein binding non-motor microtubule binding protein
          Process(es)
          G2/M transition of mitotic cell cycle apoptotic process mitotic nuclear division cell proliferation activation of protein kinase activity cell division regulation of mitotic spindle organization mitotic spindle assembly regulation of signal transduction by p53 class mediator cell cycle spindle assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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