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Immunohistochemistry analysis of TCR Alpha F1 showing staining in the cytoplasm of paraffin-treated human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a TCR Alpha F1 monoclonal antibody (Product # TCR1145) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Yeast, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human TCR alpha chain constant region|
|Storage buffer||PBS with 0.5% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 µg/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Immunoprecipitation (IP)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
TCR1145 targets TCR Alpha F1 in IHC (F), IHC (P) and IP applications and shows reactivity with Human samples.
The TCR1145 immunogen is human TCR a chain constant region.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification and engineering of human variable regions that allow expression of stable single-chain T cell receptors.
TCR1145 was used in flow cytometry to development a novel approach to stably express single-chain T cell receptors
|Aggen DH,Chervin AS,Insaidoo FK,Piepenbrink KH,Baker BM,Kranz DM||Protein engineering, design & selection : PEDS (24:361)||2011|
Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain ValphaVbeta fragments.
TCR1145 was used in flow cytometry to optimize the yeast surface display of TCRs and the scFv D1.3
|Richman SA,Aggen DH,Dossett ML,Donermeyer DL,Allen PM,Greenberg PD,Kranz DM||Molecular immunology (46:902)||2009|
A reversible functional defect of CD8+ T lymphocytes involving loss of tetramer labeling.
TCR1145 was used in flow cytometry to study the regulatory mechanism for CD8+ T lymphocyte cytolytic activity
|Demotte N,Colau D,Ottaviani S,Godelaine D,Van Pel A,Boon T,van der Bruggen P||European journal of immunology (32:1688)||2002|
Cytotoxic T cells are preferentially activated in the duodenal epithelium from patients with florid coeliac disease.
TCR1145 was used in immunohistochemistry to investigate the activation of cytotoxic T cells in duodenal epithelium from patients with florid coeliac disease
|Buri C,Burri P,Bähler P,Straumann A,Müller-Schenker B,Birrer S,Mueller C||The Journal of pathology (206:178)||2005|
Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome.
TCR1145 was used in immunohistochemistry to study the effect of CD3 surface expression on cutaneous T cell lymphoma cells
|Sano S,Matsui Y,Itami S,Yoshikawa K||Clinical and experimental immunology (113:190)||1998|
A role for the cytoplasmic tail of the pre-T cell receptor (TCR) alpha chain in promoting constitutive internalization and degradation of the pre-TCR.
TCR1145 was used in immunoprecipitation to study the mechanism for internalization and degradation of the pre-TCR molecules
|Carrasco YR,Navarro MN,Toribio ML||The Journal of biological chemistry (278:14507)||2003|
Kinetics of T-cell receptor binding by bivalent HLA-DR. Peptide complexes that activate antigen-specific human T-cells.
TCR1145 was used in western blot to investigate the role of HLA-DR2 for T-cell activation
|Appel H,Gauthier L,Pyrdol J,Wucherpfennig KW||The Journal of biological chemistry (275:312)||2000|