|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human TRADD.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is liver carcinoma.
TRADD (TNF-R1-associated Death Domain) is an important mediator of FAS and TNF-r1 associated apoptotic signaling. It contains a death domain homologous region (DDH) through which it binds to both FAS and TNF-R1. Overexpression of TRADD causes NFkB activation and apoptosis in absence of TNF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.