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Invitrogen
TRAF5 Polyclonal Antibody
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FIGURE: 1 / 1
TRAF5 Antibody (PA5-95470) in WB
Western blot analysis of TRAF5 in Lane 1: rat ovary tissue lysate, Lane 2: mouse testis tissue lysate, Lane 3: mouse thymus tissue lysate, Lane 4: human MCF-7 whole cell lysate, Lane 5: human A549 whole cell lysate, Lane 6: human HeLa whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with TRAF5 polyclonal antibody (Product # PA5-9... View More
Product Details
PA5-95470
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TRAF5 recombinant protein (Position: K277-L557).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human TRAF5 shares 82.3% amino acid (aa) sequence identity with mouse TRAF5.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat ovary tissue, mouse testis tissue, mouse thymus tissue, human MCF-7 whole cell, human A549 whole cell, human Hela whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e. g. RANK, CD30, CD40, etc. ) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor. Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymphocyte activation (5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RING finger protein 84; TNF receptor-associated factor 5; TRAF5; unnamed protein product
Gene Aliases: MGC:39780; RNF84; TRAF5
UniProt ID: (Human) O00463, (Mouse) P70191
Entrez Gene ID: (Human) 7188, (Rat) 684173, (Mouse) 22033
tumor necrosis factor receptor binding
protein binding
zinc ion binding
ubiquitin protein ligase binding
thioesterase binding
signaling adaptor activity
identical protein binding
metal ion binding
ubiquitin protein ligase activity
ubiquitin-protein transferase activity
signal transducer activity
scaffold/adaptor protein
apoptotic process
signal transduction
cell surface receptor signaling pathway
positive regulation of cell proliferation
signal transduction involved in regulation of gene expression
CD40 signaling pathway
tumor necrosis factor-mediated signaling pathway
regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
mRNA stabilization
positive regulation of NF-kappaB transcription factor activity
interleukin-17-mediated signaling pathway
protein ubiquitination
positive regulation of sequence-specific DNA binding transcription factor activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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