Invitrogen

TRAK2 Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (3)

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TRAK2 Antibody (PA5-21858) in WB

Western Blot using TRAK2 Polyclonal Antibody (Product # PA5-21858). Sample (30 µg of whole cell lysate). A: Hep G2. B: Molt-4. 7.5% SDS PAGE. TRAK2 Polyclonal Antibody (Product # PA5-21858) diluted at 1:1,000.

Product Image Gallery

TRAK2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 6 Truncated tau reduces TRAK2 expression and increases its association with mitochondrial fraction in immortalized cortical neurons. (A) Representative western blot images of CN 1.4 cells transfected with GFP, GFP-T4 (normal tau), and GFP-T4C3 (truncated tau) indicating the relative TRAK2 expression levels. Caspase-cleaved tau decreases TRAK2 expression compared with cells that were expressing GFP or GFP-T4 (normal tau). (A') Quantitative analysis of the relative expression levels of TRAK2 in immortalized neurons transfected with indicated conditions. Data are mean +- SE, n = 5. * p < 0.05 indicates differences between groups calculated by ANOVA test. (A'') Total RNA was extracted from immortalized cortical neurons transfected with GFP, GFP-T4 (full-length tau), and GFP-T4C3 (truncated tau) and mRNA expression levels of TRAK2 were measured by RT-PCR. Data represent mean +- SE, n = 3. (B) Representative western blot images of CN 1.4 cells for mitochondrial extracts transfected with the conditions indicated. (B') Quantitative graph showing that caspase-cleaved tau expression increases TRAK2 presence in mitochondrial extracts compared with cells transfected with the full-length tau form (GFP-T4). Data are mean +- SE, n = 4. * p < 0.05 indicates differences between groups calculated by ANOVA test. (C) Representative western blot images and quantitative analysis (C') of kinesin 1 protein levels in mitochondrial extracts of CN 1.4 cells transfected with the conditions indica

Product Details

PA5-21858

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 852 and 914 of TRAK2 (Uniprot ID#O60296)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11155420

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

Predicted reactivity: Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

Published figure using TRAK2 polyclonal antibody (Product # PA5-21858) in Western Blot

Mon Sep 28 00:00:00 EDT 2020

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

Published figure using TRAK2 polyclonal antibody (Product # PA5-21858) in Western Blot

Mon Sep 28 00:00:00 EDT 2020

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

Published figure using TRAK2 polyclonal antibody (Product # PA5-21858) in Western Blot

Mon Sep 28 00:00:00 EDT 2020

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells.

Frontiers in cellular neuroscience

Quintanilla RA,Tapia-Monsalves C,Vergara EH,Pérez MJ,Aranguiz A

Published figure using TRAK2 polyclonal antibody (Product # PA5-21858) in Western Blot

Mon Sep 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 3; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; EMBL AAH60681.1; gamma-aminobutyric acid(A) receptor-interacting factor; milton homolog 2; O-linked N-acetylglucosamine transferase interacting protein 98; Trafficking kinesin-binding protein 2; trafficking protein, kinesin binding 2

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Gene Aliases: ALS2CR3; CALS-C; GRIF-1; GRIF1; KIAA0549; MILT2; OIP98; TRAK2

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UniProt ID: (Human) O60296

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Entrez Gene ID: (Human) 66008

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Molecular Function: membrane traffic protein

Function(s)

regulation of transcription from RNA polymerase II promoter protein O-linked glycosylation protein targeting

Process(es)

receptor binding protein binding enzyme binding GABA receptor binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TRAK2 Polyclonal Antibody

TRAK2 Antibody (PA5-21858) in WB

Product Details

PA5-21858

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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