Immunofluorescent analysis of TRAK2 showing staining in the nucleus and cytoplasm of A375 cells. A375 cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min and stained using a TRAK2 polyclonal antibody (Product # PA5-34889) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contains a sequence corresponding to a region within amino acids 630 and 914 of TRAK2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34889 targets TRAK2 in WB applications and shows reactivity with Human and Mouse samples.
The PA5-34889 immunogen is recombinant protein fragment contains a sequence corresponding to a region within amino acids 630 and 914 of TRAK2.
May regulate endosome-to-lysosome trafficking of membrane cargo, including EGFR.
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