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Invitrogen
TRAP220 Polyclonal Antibody
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Product Details
PA5-37384
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length human TRAP220 / MED1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 170 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; nuclear receptor interacting protein; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; PPARG binding protein; PPRB; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; unnamed protein product; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205
Gene Aliases: ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
UniProt ID: (Human) Q15648
Entrez Gene ID: (Human) 5469
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription coactivator binding
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
protein binding
ligand-dependent nuclear receptor binding
estrogen receptor binding
chromatin DNA binding
histone acetyltransferase binding
vitamin D receptor binding
retinoic acid receptor binding
peroxisome proliferator activated receptor binding
macromolecular complex binding
thyroid hormone receptor binding
LBD domain binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitin protein ligase activity
general transcription initiation factor binding
DNA-binding transcription factor binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
angiogenesis
in utero embryonic development
liver development
embryonic placenta development
lens development in camera-type eye
thyroid hormone mediated signaling pathway
ventricular trabecula myocardium morphogenesis
retinal pigment epithelium development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
thyroid hormone generation
protein import into nucleus
androgen biosynthetic process
brain development
heart development
lactation
positive regulation of cell proliferation
organ morphogenesis
positive regulation of gene expression
negative regulation of keratinocyte proliferation
protein ubiquitination
keratinocyte differentiation
monocyte differentiation
intracellular steroid hormone receptor signaling pathway
organ regeneration
positive regulation of transcription elongation from RNA polymerase II promoter
positive regulation of intracellular estrogen receptor signaling pathway
mammary gland epithelial cell proliferation
embryonic heart tube development
embryonic hindlimb morphogenesis
embryonic hemopoiesis
peroxisome proliferator activated receptor signaling pathway
cellular response to hepatocyte growth factor stimulus
megakaryocyte development
mRNA transcription from RNA polymerase II promoter
camera-type eye development
negative regulation of apoptotic process
G0 to G1 transition
fat cell differentiation
positive regulation of keratinocyte differentiation
positive regulation of erythrocyte differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
erythrocyte development
enucleate erythrocyte development
RNA polymerase II transcriptional preinitiation complex assembly
hematopoietic stem cell differentiation
positive regulation of transcription initiation from RNA polymerase II promoter
positive regulation of interferon-gamma-mediated signaling pathway
mammary gland branching involved in thelarche
mammary gland branching involved in pregnancy
epithelial cell proliferation involved in mammary gland duct elongation
positive regulation of G0 to G1 transition
regulation of vitamin D receptor signaling pathway
cellular response to epidermal growth factor stimulus
cellular response to steroid hormone stimulus
cellular response to thyroid hormone stimulus
positive regulation of hepatocyte proliferation
regulation of RNA biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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