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Invitrogen
TRIB1 Polyclonal Antibody
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FIGURE: 1 / 1
TRIB1 Antibody (PA5-87662) in WB
Western blot analysis of TRIB1 in extracts of various cell lines. Samples were incubated with TRIB1 Polyclonal antibody (Product # PA5-87662) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-87662
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 203-372 of human TRIB1 (NP_0794711)
Conjugate
Form
Liquid
Concentration
3.35 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LGDLKLRKFV FSTEERTQLR LESLEDTHIM KGEDDALSDK HGCPAYVSPE ILNTTGTYSG KAADVWSLGV MLYTLLVGRY PFHDSDPSAL FSKIRRGQFC IPEHISPKAR CLIRSLLRRE PSERLTAPEI LLHPWFESVL EPGYIDSEIG TSDQIVPEYQ EDSDISSFFC; Positive Samples: U-87MG, BxPC-3, Raji
Target Information
TRIB1 also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities. Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G-protein-coupled receptor induced protein; G-protein-coupled receptor-induced gene 2 protein; G-protein-coupled receptor-induced protein 2; GIG-2; phosphoprotein regulated by mitogenic pathways; SKIP1; TRB-1; TRIB1; Tribbles homolog 1; tribbles-like protein 1
Gene Aliases: C8FW; GIG-2; GIG2; SKIP1; TRB-1; TRB1; TRIB1
UniProt ID: (Human) Q96RU8
Entrez Gene ID: (Human) 10221
protein phosphorylation
negative regulation of protein kinase activity
JNK cascade
negative regulation of smooth muscle cell migration
negative regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
response to lipopolysaccharide
regulation of MAP kinase activity
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of eosinophil differentiation
positive regulation of macrophage differentiation
negative regulation of neutrophil differentiation
negative regulation of smooth muscle cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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