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Invitrogen
TRIB3 Polyclonal Antibody
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FIGURE: 1 / 2
TRIB3 Antibody (PA5-75683) in ICC/IF
Immunofluorescence analysis of Tribbles homolog 3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with TRIB3 Polyclonal Antibody (Product # PA5-75683) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More
Product Details
PA5-75683
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues in Human TRIB3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
A novel inhibitor of cell division, Tribbles, blocks mitosis at a critical point in Drosophila morphogenesis. Recently, mammalian homologues of Tribbles (Trbs) have been identified that control mitogen-activated protein kinase (MAPK) activity. TRIB3 also known as TRB-3, SINK, and SKIP3 is a member of Trbs family. Overexpression of this protein inhibits NF-kB-dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but does not inhibit NF-kB translocation to the nucleus and binding to DNA. TRIB3/SKIP3 is also overexpressed in multiple human tumors and is regulated by hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C20orf97; DJ1103G7.3; induced in fatty liver dystrophy 2; kinase; Neuronal cell death Inducible Putative Kinase; Neuronal cell death-inducible putative kinase; p65-interacting inhibitor of NF-kappa-B; p65-interacting inhibitor of NF-kappaB; SINK; TRB-3; Tribbles 3; Tribbles homolog 3; unnamed protein product
Gene Aliases: C20orf97; Ifld2; NIPK; SINK; SKIP3; TRB-3; TRB3; TRIB3
UniProt ID: (Human) Q96RU7, (Mouse) Q8K4K2, (Rat) Q9WTQ6
Entrez Gene ID: (Human) 57761, (Mouse) 228775, (Rat) 246273
transcription corepressor activity
protein kinase inhibitor activity
protein binding
ATP binding
kinase activity
enzyme binding
protein kinase binding
protein serine/threonine kinase inhibitor activity
mitogen-activated protein kinase kinase binding
ubiquitin protein ligase binding
ubiquitin-protein transferase regulator activity
ubiquitin ligase activator activity
protein kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
negative regulation of transcription from RNA polymerase II promoter
apoptotic process
regulation of autophagy
regulation of glucose transport
positive regulation of protein ubiquitination
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to insulin stimulus
response to endoplasmic reticulum stress
regulation of MAP kinase activity
negative regulation of MAPK cascade
negative regulation of fat cell differentiation
negative regulation of fatty acid biosynthetic process
negative regulation of transcription, DNA-templated
negative regulation of insulin receptor signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
positive regulation of protein binding
positive regulation of protein catabolic process
positive regulation of ubiquitin-protein transferase activity
negative regulation of nucleic acid-templated transcription
programmed cell death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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