Invitrogen

TRIM Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (4) TRIM antibodies

Datasheet

Datasheet

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TRIM Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of TRIM in human lymph node and cerebral cortex tissues using TRIM Polyclonal Antibody (Product # PA5-51706). Corresponding TRAT1 RNA-seq data are presented for the same tissues.

TRIM Antibody in Western Blot (WB) — Western blot analysis of TRIM in control (vector only transfected HEK293T lysate) and TRAT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a TRIM Polyclonal Antibody (Product # PA5-51706).

TRIM Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of TRIM demonstrates antibody specificity. Immunohistochemical analysis of TRIM using anti-TRIM Polyclonal Antibody (Product # PA5-51706), shows significant staining of TRIM in human lymph node and shows minimal or weak staining in human cerebral cortex tissues. The relative expression levels of TRIM within each tissue is shown using RNA-Seq. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human TRIM

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2648908

Product Specific Information

Immunogen sequence: RQDKMYSYSS DHTRVDEYYI EDTPIYGNLD DMISEPMDEN CYEQMKARPE KSVNKMQEAT PSAQATNETQ MCYASLDHSV KGKRRKPRKQ NTHFSDKDGD EQLHAI

Highest antigen sequence identity to the following orthologs: Mouse - 62%, Rat - 58%.

Target Information

TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HSPC062; pp29/30; T cell receptor interacting molecule; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; TCRIM; TRIM

Gene Aliases: HSPC062; pp29/30; TCRIM; TRAT1; TRIM

UniProt ID: (Human) Q6PIZ9

Entrez Gene ID: (Human) 50852

Function(s)