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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 19 amino acid peptide from near the carboxy terminus of human TRIM25.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 µg/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is Hela cell lysate.
PA5-20410 can be used with blocking peptide PEP-0527.
TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
E3 ubiquitin/ISG15 ligase TRIM25; EFP, RNF147, Z147, ZNF147; estrogen-responsive finger protein; RING finger protein 147; RING-type E3 ubiquitin transferase; tripartite motif protein 25; tripartite motif protein TRIM25; tripartite motif-containing 25; tripartite motif-containing protein 25; ubiquitin/ISG15-conjugating enzyme TRIM25; zinc finger protein 147; zinc finger protein 147 (estrogen-responsive finger protein); zinc finger protein-147
AA960166; AL022677; EFP; RNF147; TRIM25; Z147; Zfp147; ZNF147