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Invitrogen
TRIM28 Polyclonal Antibody
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FIGURE: 1 / 8
TRIM28 Antibody (PA5-34485) in ICC/IF
Immunofluorescence analysis of TRIM28 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIM28 Polyclonal Antibody (Product # PA5-34485) at 5µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
Product Details
PA5-34485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 14 amino acid peptide near the carboxy terminus of human TRIM28
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human testis tissue lysate.
PA5-34485 can be used with blocking peptide PEP-1527.
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28; unnamed protein product
Gene Aliases: AA408787; KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; TIF1beta; TRIM28
UniProt ID: (Human) Q13263, (Mouse) Q62318, (Rat) O08629
Entrez Gene ID: (Human) 10155, (Mouse) 21849, (Rat) 116698
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
RNA binding
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
SUMO transferase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
metal ion binding
ubiquitin protein ligase activity
ubiquitin-like protein ligase activity
chromo shadow domain binding
promoter-specific chromatin binding
transcription factor activity, sequence-specific DNA binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
sequence-specific DNA binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
poly(A) RNA binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
epithelial to mesenchymal transition
DNA repair
chromatin organization
methylation-dependent chromatin silencing
embryo implantation
positive regulation of macromolecule metabolic process
protein sumoylation
positive regulation of protein import into nucleus
DNA methylation involved in embryo development
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation by host of viral release from host cell
innate immune response
positive regulation of DNA repair
negative regulation of single stranded viral RNA replication via double stranded DNA intermediate
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of nucleobase-containing compound metabolic process
convergent extension involved in axis elongation
embryonic placenta morphogenesis
genetic imprinting
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
protein ubiquitination
positive regulation of transcription factor import into nucleus
protein autophosphorylation
protein oligomerization
negative regulation of DNA demethylation
negative regulation of viral release from host cell
regulation of genetic imprinting
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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