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TRIM6 Antibody (PA5-143867) in ICC/IF
Immunocytochemistry analysis of TRIM6 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TRIM6 Polyclonal antibody (Product # PA5-143867) using a dilution of 2 μg/mL. DyLight®488 conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143867
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TRIM6 recombinant protein (Position: Q50-H349).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075081
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K56 whole cell, human HEK293 whole cell, human A549 whole cell, rat thymus tissue, mouse thymus tissue. IHC: human tonsil tissue, mouse thymus tissue, rat spleen tissue. ICC/IF: U20S cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
TRIM6 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A read-through transcript transcribed from this gene and TRIM34 has been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; tripartite motif protein 6; tripartite motif-containing 6; Tripartite motif-containing protein 6
Gene Aliases: C430046K18Rik; D7Ertd684e; RNF89; TRIM6
UniProt ID: (Human) Q9C030, (Mouse) Q8BGE7
Entrez Gene ID: (Human) 117854, (Rat) 293294, (Mouse) 94088
protein polyubiquitination
positive regulation of defense response to virus by host
positive regulation of cytokine secretion involved in immune response
positive regulation of gene expression
negative regulation of gene expression
positive regulation of peptidyl-threonine phosphorylation
free ubiquitin chain polymerization
response to lipopolysaccharide
positive regulation of peptidyl-serine phosphorylation
cellular response to interferon-beta
negative regulation of viral genome replication
negative regulation of transcription, DNA-templated
positive regulation of type I interferon-mediated signaling pathway
protein trimerization
cellular response to virus
regulation of NIK/NF-kappaB signaling
positive regulation of transcription regulatory region DNA binding
negative regulation of stem cell differentiation
biological_process
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