Invitrogen

TRIM71 Polyclonal Antibody

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TRIM71 Antibody in Western Blot (WB) — Western Blot staining of NIH/3T3 cell lysate using Product # PA5-19282 at a concentration of 0.5 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Product Details

PA5-19282

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (NKLESTISAVQ ) corresponding to the internal amino acids of TRIM71 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10977393

Product Specific Information

This antibody is predicted to react with bovine, canine, human and rat based on sequence homology.

Target Information

TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dual analysis of the murine cytomegalovirus and host cell transcriptomes reveal new aspects of the virus-host cell interface.

PLoS pathogens

Juranic Lisnic V,Babic Cac M,Lisnic B,Trsan T,Mefferd A,Das Mukhopadhyay C,Cook CH,Jonjic S,Trgovcich J

PA5-19282 was used in western blot to determine the roles of murine cytomegalovirus and host cell transcriptomes show new aspects of the virus-host cell interface

Mon Apr 28 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase TRIM71; lin-41 homolog; mLin41; Protein lin-41 homolog; RING-type E3 ubiquitin transferase TRIM71; Tripartite motif-containing protein 71

Gene Aliases: 2610206G21Rik; AL022943; Gm1127; lin-41; Lin41; mlin-41; mLin41; Trim71

UniProt ID: (Mouse) Q1PSW8

Entrez Gene ID: (Mouse) 636931

Molecular Function: ubiquitin-protein ligase

Function(s)

G1/S transition of mitotic cell cycle neural tube closure multicellular organism development fibroblast growth factor receptor signaling pathway miRNA metabolic process negative regulation of translation neural tube development gene silencing by RNA miRNA mediated inhibition of translation regulation of protein metabolic process protein autoubiquitination regulation of gene silencing by miRNA 3'-UTR-mediated mRNA destabilization cellular response to organic substance stem cell proliferation regulation of neural precursor cell proliferation positive regulation of gene silencing by miRNA

Process(es)

RNA binding ubiquitin-protein transferase activity protein binding zinc ion binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity translation repressor activity miRNA binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding ubiquitin protein ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

TRIM71 Polyclonal Antibody

Product Details

PA5-19282

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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