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TRIM9 Antibody (PA5-89513) in WB
Western blot analysis of TRIM9 in extracts of various cell lines. Samples were incubated with TRIM9 Polyclonal antibody (Product # PA5-89513) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89513
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TRIM9 (NP_0559784)
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805622
Product Specific Information
Immunogen sequence: MEEMEEELKC PVCGSFYREP IILPCSHNLC QACARNILVQ TPESESPQSH RAAGSGVSDY DYLDLDKMSL YSEADSGYGS YGGFASAPTT PCQKSPNGVR VFPPAMPPPA THLSPALAPV PRNSCITCPQ CHRSLILDDR GLRGFPKNRV LEGVIDRYQQ; Positive Samples: HeLa, HepG2, DU145, Mouse brain, Rat brain; Cellular Location: Cell junction, Cell projection, Cytoplasm, Cytoplasmic vesicle, cytoskeleton, dendrite, secretory vesicle, synapse, synaptic vesicle
Target Information
TRIM9 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms. The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternate splicing of this gene generates two transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase TRIM9; homolog of rat RING finger Spring; RING finger protein 91; RING-type E3 ubiquitin transferase TRIM9; SNAP-25-interacting RING finger protein; Spring; tripartite motif protein 9; tripartite motif-containing 9; Tripartite motif-containing protein 9
Gene Aliases: KIAA0282; RNF91; SPRING; TRIM9
UniProt ID: (Human) Q9C026, (Mouse) Q8C7M3, (Rat) Q91ZY8
Entrez Gene ID: (Human) 114088, (Mouse) 94090, (Rat) 155812
SNARE binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
protein homodimerization activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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