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Invitrogen
TRPC6 Polyclonal Antibody
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FIGURE: 1 / 3
TRPC6 Antibody (PA5-28100) in IHC (P)
Immunohistochemistry (Paraffin) analysis of TRPC6 was performed in paraffin-embedded mouse cerebellum tissue using Green: TRPC6 Polyclonal Antibody (Product # PA5-29847) at a dilution of 1:500. Red: NF-H, a neuron marker, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-28100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human TRPC6. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 40% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji, A549, A549 membrane fraction extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of the Trpc6 channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Further, Trpc6 is a member of the mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the 'canonical TRP' (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, Trpc6 acts as a non-selective store-independent receptor-activated cation channel. Trpc6 is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium entry channel; FLJ11098; FLJ14863; focal segmental glomerulosclerosis 2; HGNC:12338; Short transient receptor potential channel 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; TRP-6; trp6A; TrpC6
Gene Aliases: AV025995; FSGS2; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; TRP6; TRPC6; Trrp6
UniProt ID: (Human) Q9NQA9, (Mouse) Q61143
Entrez Gene ID: (Human) 7225, (Mouse) 22068
cation transport
manganese ion transport
positive regulation of cytosolic calcium ion concentration
single fertilization
aging
positive regulation of peptidyl-threonine phosphorylation
platelet activation
neuron differentiation
positive regulation of ion transmembrane transporter activity
positive regulation of neuron differentiation
negative regulation of dendrite morphogenesis
regulation of cytosolic calcium ion concentration
positive regulation of calcium ion transport
cellular response to hydrogen peroxide
calcium ion transmembrane transport
cellular response to hypoxia
transport
ion transport
calcium ion transport
transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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