Invitrogen

TRPM2 Polyclonal Antibody

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TRPM2 Antibody in Western Blot (WB) — Western Blot analysis of TRPM2 was performed by separating 30 µg of HepG2 whole cell and membrane extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRPM2 Polyclonal Antibody (Product # PA5-22218) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

TRPM2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of TRPM2 in paraffin-embedded MDA-MB-468 xenograft using a TRPM2 polyclonal antibody (Product # PA5-22218) at a dilution of 1:100.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 181 and 423 of TRPM2 (Uniprot ID#O94759)

Conjugate

Unconjugated

Form

Liquid

Concentration

1.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152374

Product Specific Information

Recommended positive controls: HepG2, HepG2 membrane extract.

Predicted reactivity: Mouse (90%), Rat (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TRPM2 belongs to the ion transport protein family which includes Sodium, Potassium, Calcium ion channels. This family is characterized by proteins possessing 6 transmembrane helices in which the last two helices flank a loop which determines ion selectivity. TRPM2 is activated by oxidative stress and confers susceptibility to cell death. Further, TRPM2 is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. Several alternatively spliced transcript variants of the TRPM2 gene have been described, but their full-length nature is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 3.6.1.13; EREG 1; Estrogen-responsive element-associated gene 1 protein; KNP 3; Long transient receptor potential channel 2; LTRPC 2; LTrpC-2; MGC133383; null; Transient receptor potential cation channel subfamily M member 2; transient receptor potential cation channel, subfamily M, member 2; Transient receptor potential channel 7; Transient receptor potential melastatin 2; transient receptor potential melastatin family 2; TRPC 7; TrpC7; TRPM 2

Gene Aliases: EREG1; KNP3; LTrpC-2; LTRPC2; NUDT9H; NUDT9L1; TRPC7; TRPM2; Trpm2-predicted

UniProt ID: (Human) O94759, (Rat) E9PTA2

Entrez Gene ID: (Human) 7226, (Rat) 294329

Molecular Function: ion channel receptor transporter