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          • Primary Antibodies ›
          • TRUB2 Antibodies

          Invitrogen

          TRUB2 Polyclonal Antibody

          View all (7) TRUB2 antibodies

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          Cite TRUB2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          TRUB2 Antibody in Western Blot (WB)
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          TRUB2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          TRUB2 Antibody (PA5-90731) in WB

          Western blot analysis of TRUB2 in extracts of various cell lines. Samples were incubated with TRUB2 Polyclonal antibody (Product # PA5-90731) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TRUB2 Antibody in Western Blot (WB)
          TRUB2 Polyclonal Antibody

          Product Details

          PA5-90731

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 122-331 of human TRUB2 (NP_0564941)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.11 mg/mL

          Amount

          111 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806209

          Product Specific Information

          Immunogen sequence: AHLTKDYTVR GLLGKATDDF REDGRLVEKT TYDHVTREKL DRILAVIQGS HQKALVMYSN LDLKTQEAYE MAVRGLIRPM NKSPMLITGI RCLYFAPPEF LLEVQCMHET QKELRKLVHE IGLELKTTAV CTQVRRTRDG FFTLDSALLR TQWDLTNIQD AIRAATPQVA AELEKSLSPG LDTKQLPSPG WSWDSQGPSS TLGLERGAGQ; Positive Samples: K-562, HeLa

          Target Information

          Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: mitochondrial mRNA pseudouridine synthase TRUB2; probable tRNA pseudouridine synthase 2; Pseudouridylate synthase TRUB2, mitochondrial; Psi55 synthase TRUB2; RP11-339B21.1; tRNA pseudouridine 55 synthase TRUB2; TruB pseudouridine (psi) synthase family member 2; TruB pseudouridine (psi) synthase homolog 2; TruB pseudouridine synthase homolog 2; unnamed protein product

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          Gene Aliases: CLONE24922; TRUB2

          View more View less

          UniProt ID: (Human) O95900

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          Entrez Gene ID: (Human) 26995

          View more View less

          Function(s)
          RNA binding pseudouridine synthase activity isomerase activity tRNA pseudouridine(55) synthase activity
          Process(es)
          pseudouridine synthesis RNA processing mRNA processing tRNA processing RNA modification positive regulation of mitochondrial translation mRNA pseudouridine synthesis
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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