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TSC1 Antibody (PA5-116079) in ICC/IF
Immunocytochemistry analysis of TSC1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116079) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116079
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TSC1(Accession Q92574), corresponding to amino acid residues L1121-S1164.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TSC1.
Target Information
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor
Gene Aliases: KIAA0243; LAM; TSC; TSC1
UniProt ID: (Human) Q92574, (Rat) Q9Z136, (Mouse) Q9EP53
Entrez Gene ID: (Human) 7248, (Rat) 60445, (Mouse) 64930
kidney development
neural tube closure
regulation of cell-matrix adhesion
adaptive immune response
rRNA export from nucleus
regulation of translation
potassium ion transport
cell cycle arrest
cell-matrix adhesion
negative regulation of cell proliferation
adult locomotory behavior
negative regulation of macroautophagy
negative regulation of translation
hippocampus development
cerebral cortex development
cell projection organization
negative regulation of TOR signaling
response to insulin
myelination
memory T cell differentiation
regulation of phosphoprotein phosphatase activity
negative regulation of cell size
regulation of protein kinase activity
glucose import
negative regulation of insulin receptor signaling pathway
synapse organization
protein stabilization
protein heterooligomerization
regulation of stress fiber assembly
regulation of cell cycle
positive regulation of focal adhesion assembly
cardiac muscle cell differentiation
activation of GTPase activity
cellular response to oxygen-glucose deprivation
regulation of neuron death
nervous system development
regulation of actin cytoskeleton organization
regulation of focal adhesion assembly
embryo development
regulation of GTPase activity
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