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Invitrogen

TSC2 Polyclonal Antibody

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TSC2 Polyclonal Antibody

Product Details

PA5-95194

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Tuberin recombinant protein (Position: H1611-V1807).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2806999

Product Specific Information

Human Tuberin shares 94% and 90% amino acid (aa) sequences identity with mouse and rat Tuberin, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human U20S whole cell, human PANC whole cell, human HepG2 whole cell, human A549 whole cell, human COLO320 whole cell. IHC: rat brain tissue, mouse brain tissue, human mammary cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000198394; protein phosphatase 1, regulatory subunit 160; renal carcinoma; Tsc2; Tuberin; tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; Tuberous sclerosis 2 protein homolog; tuberous sclerosis complex locus 2; unnamed protein product

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Gene Aliases: LAM; Nafld; PPP1R160; Rc; Tcs2; TSC2; TSC4

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UniProt ID: (Human) P49815, (Rat) P49816

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Entrez Gene ID: (Human) 7249, (Rat) 24855, (Mouse) 22084

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Function(s)
GTPase activator activity protein binding phosphatase binding small GTPase binding protein homodimerization activity Hsp90 protein binding protein heterodimerization activity 14-3-3 protein binding GTPase-activating protein
Process(es)
neural tube closure cytoplasmic translation protein import into nucleus endocytosis heart development protein localization negative regulation of cell proliferation cellular response to starvation vesicle-mediated transport positive regulation of macroautophagy regulation of macroautophagy regulation of endocytosis negative regulation of Wnt signaling pathway cellular response to nutrient levels negative regulation of TOR signaling TORC1 signaling anoikis negative regulation of translational initiation positive regulation of translational initiation regulation of insulin receptor signaling pathway negative regulation of insulin receptor signaling pathway positive chemotaxis regulation of small GTPase mediated signal transduction regulation of cell cycle negative regulation of protein kinase B signaling negative regulation of macromitophagy negative regulation of TORC1 signaling positive regulation of TORC1 signaling response to hypoxia negative regulation of protein kinase activity acute-phase response signal transduction phosphatidylinositol 3-kinase signaling negative regulation of phosphatidylinositol 3-kinase signaling cell projection organization cellular response to insulin stimulus negative regulation of MAP kinase activity protein kinase B signaling positive regulation of GTPase activity protein transport into plasma membrane raft establishment of protein localization negative regulation of cell size positive regulation of transcription from RNA polymerase II promoter glucose import insulin-like growth factor receptor signaling pathway negative regulation of epithelial cell proliferation protein homooligomerization protein heterooligomerization
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