Bethyl Laboratories

TSG101 Polyclonal Antibody

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TSG101 Antibody in Western Blot (WB) — **Detection of human TSG101 by western blot.** *Samples:* Whole cell lysate from HeLa (15 and 50 µg), 293T (T; 50 µg) and Jurkat (J; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-TSG101 antibody A303-506A used for WB at 0.04 µg/ml. *Detection:* Chemiluminescence with an exposure time of 3 minutes.

Product Details

A303-506A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 225 and 275 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.20 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

Gene Aliases: TSG10; TSG101; VPS23

UniProt ID: (Human) Q99816

Entrez Gene ID: (Human) 7251

Molecular Function: ubiquitin-protein ligase