Invitrogen

TSG101 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

10 References

View all (26) TSG101 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

TSG101 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — TSG101 Polyclonal Antibody detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 Polyclonal Antibody (Product # PA5-31260) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

TSG101 Antibody in Western Blot (WB) — Western Blot using TSG101 Polyclonal Antibody (Product # PA5-31260). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 Polyclonal Antibody (Product # PA5-31260) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

TSG101 Antibody in ELISA (ELISA) — ELISA detection of TSG101 using TSG101 Polyclonal Antibody (Product # PA5-31260) for capture at a concentration of 5 µg/mL and TSG101 Monoclonal Antibody (4A10) (Product # MA1-23296) for detection at a concentration of 1.5 µg/mL.

TSG101 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of the target protein. 293T cells were transfected with TSG101 shRNA and decrease in signal intensity was observed in western blot application using TSG101 Polyclonal Antibody (Product # PA5-31260). {KD}

Product Details

PA5-31260

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
ELISA (ELISA)	1:1,000-1:10,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Bovine, Horse, Human, Mouse, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein fragment corresponding to a region within amino acids 117 and 324 of Human TSG101 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.09 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2548734

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics.

Frontiers in aging neuroscience

Cazzaro S,Fang C,Khan H,Witas R,Kee TR,Woo JA,Kang DE

PA5-31260 was used in Western Blot to suggest that increasing cargo receptor engagement with autophagic cargo and reducing actin dynamics (i.e., SSH1 inhibition) represents an attractive strategy to promote misfolded protein degradation while reducing sEV-mediated cell to cell spread of pathology.

Tue Sep 13 00:00:00 EDT 2022

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Mon Mar 07 00:00:00 EST 2022

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Mon Mar 07 00:00:00 EST 2022

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Mon Mar 07 00:00:00 EST 2022

Small Extracellular Vesicles in Pre-Therapy Plasma Predict Clinical Outcome in Non-Small-Cell Lung Cancer Patients.

Cancers

Vetsika EK,Sharma P,Samaras I,Markou A,Georgoulias V,Whiteside TL,Kotsakis A

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Apr 23 00:00:00 EDT 2021

Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression.

Journal of extracellular vesicles

Pietrowska M,Zebrowska A,Gawin M,Marczak L,Sharma P,Mondal S,Mika J,Polańska J,Ferrone S,Kirkwood JM,Widlak P,Whiteside TL

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.

International journal of molecular sciences

Almiñana C,Rudolf Vegas A,Tekin M,Hassan M,Uzbekov R,Fröhlich T,Bollwein H,Bauersachs S

PA5-31260 was used in Western Blotting to characterize extracellular vesicles and their role in embryo-maternal dialogue in mares.

Tue Jan 19 00:00:00 EST 2021

Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.

International journal of molecular sciences

Almiñana C,Rudolf Vegas A,Tekin M,Hassan M,Uzbekov R,Fröhlich T,Bollwein H,Bauersachs S

PA5-31260 was used in Western Blotting to characterize extracellular vesicles and their role in embryo-maternal dialogue in mares.

Tue Jan 19 00:00:00 EST 2021

Induction of a proliferative response in the zebrafish retina by injection of extracellular vesicles.

Experimental eye research

Didiano D,Abner JJ,Hinger SA,Flickinger Z,Kent M,Clement MA,Balaiya S,Liu Q,Dai X,Levine EM,Patton JG

PA5-31260 was used in Western Blot to identify key factors that might target EVs to MG and initiate retina regeneration.

Sun Nov 01 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

PA5-31260 was used in Western Blotting to isolate and analyse peripheral B cells by flow cytometry to understand the effects of exosomes on B cells.

Wed Jul 29 00:00:00 EDT 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

PA5-31260 was used in Western Blotting to isolate and analyse peripheral B cells by flow cytometry to understand the effects of exosomes on B cells.

Wed Jul 29 00:00:00 EDT 2020

The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.

International journal of molecular sciences

Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN

PA5-31260 was used in Western Blotting to demonstrate that exosomes have the potential to serve as easily accessible, non-invasive biomarkers for tumor status and tumor aggressiveness as well as for the degree of immune suppression in HNSCC patients.

Tue May 26 00:00:00 EDT 2020

The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.

International journal of molecular sciences

Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN

Tue May 26 00:00:00 EDT 2020

JC Virus infected choroid plexus epithelial cells produce extracellular vesicles that infect glial cells independently of the virus attachment receptor.

PLoS pathogens

O'Hara BA,Morris-Love J,Gee GV,Haley SA,Atwood WJ

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Sun Mar 01 00:00:00 EST 2020

Enhanced generation of intraluminal vesicles in neuronal late endosomes in the brain of a Down syndrome mouse model with endosomal dysfunction.

Developmental neurobiology

D'Acunzo P,Hargash T,Pawlik M,Goulbourne CN,Pérez-González R,Levy E

PA5-31260 was used in Western Blotting to ivestigate the regulation of exosome secretion in Down syndrome and Alzheimer's postmortem brains in both human and mouse models.

Mon Jul 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

Gene Aliases: AI255943; CC2; TSG10; TSG101; VPS23

UniProt ID: (Human) Q99816, (Mouse) Q61187

Entrez Gene ID: (Human) 7251, (Mouse) 22088

Molecular Function: ubiquitin-protein ligase