Invitrogen

TSG101 Polyclonal Antibody

TSG101 Antibody in Immunocytochemistry (ICC/IF)

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TSG101 Antibody (PA5-31260) in ICC/IF

Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with TSG101 Polyclonal Antibody (Product # PA5-31260, 1:100( in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:200... View More

TSG101 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TSG101 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA5-31260

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100

ELISA (ELISA)

1:1,000-1:10,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Horse, Human, Mouse, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 117 and 324 of Human TSG101

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.42 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2548734

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Apolipoprotein E2 Expression Alters Endosomal Pathways in a Mouse Model With Increased Brain Exosome Levels During Aging.

Traffic (Copenhagen, Denmark)

Peng KY,Liemisa B,Pasato J,D'Acunzo P,Pawlik M,Heguy A,Penikalapati SC,Labuza A,Pidikiti H,Alldred MJ,Ginsberg SD,Levy E,Mathews PM

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed May 01 00:00:00 UTC 2024

Circadian Rhythm Does Not Affect the miRNA Cargo of Bovine Raw Milk Extracellular Vesicles.

International journal of molecular sciences

Saenz-de-Juano MD,Silvestrelli G,Ulbrich SE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Jun 16 00:00:00 UTC 2023

Small extracellular vesicle-loaded bevacizumab reduces the frequency of intravitreal injection required for diabetic retinopathy.

Theranostics

Reddy SK,Ballal AR,Shailaja S,Seetharam RN,Raghu CH,Sankhe R,Pai K,Tender T,Mathew M,Aroor A,Shetty AK,Adiga S,Devi V,Muttigi MS,Upadhya D

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed May 10 00:00:00 UTC 2023

Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients.

Molecular medicine (Cambridge, Mass.)

Tengler L,Schütz J,Tiedtke M,Jablonska J,Theodoraki MN,Nitschke K,Weiß C,Seiz E,Affolter A,Jungbauer F,Lammert A,Rotter N,Ludwig S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed May 24 00:00:00 UTC 2023

Plasma-derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients.

Molecular medicine (Cambridge, Mass.)

Tengler L,Schütz J,Tiedtke M,Jablonska J,Theodoraki MN,Nitschke K,Weiß C,Seiz E,Affolter A,Jungbauer F,Lammert A,Rotter N,Ludwig S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed May 24 00:00:00 UTC 2023

Modulation of PD‑L1 expression by standard therapy in head and neck cancer cell lines and exosomes.

International journal of oncology

Affolter A,Liebel K,Tengler L,Seiz E,Tiedtke M,Azhakesan A,Schütz J,Theodoraki MN,Kern J,Ruder AM,Fleckenstein J,Weis CA,Bieback K,Kramer B,Lammert A,Scherl C,Rotter N,Ludwig S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Sep 01 00:00:00 UTC 2023

Modulation of PD‑L1 expression by standard therapy in head and neck cancer cell lines and exosomes.

International journal of oncology

Affolter A,Liebel K,Tengler L,Seiz E,Tiedtke M,Azhakesan A,Schütz J,Theodoraki MN,Kern J,Ruder AM,Fleckenstein J,Weis CA,Bieback K,Kramer B,Lammert A,Scherl C,Rotter N,Ludwig S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Sep 01 00:00:00 UTC 2023

Arginase-1 in Plasma-Derived Exosomes as Marker of Metastasis in Patients with Head and Neck Squamous Cell Carcinoma.

Cancers

Hofmann L,Harasymczuk M,Huber D,Szczepanski MJ,Dworacki G,Whiteside TL,Theodoraki MN

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Thu Nov 16 00:00:00 UTC 2023

Arginase-1 in Plasma-Derived Exosomes as Marker of Metastasis in Patients with Head and Neck Squamous Cell Carcinoma.

Cancers

Hofmann L,Harasymczuk M,Huber D,Szczepanski MJ,Dworacki G,Whiteside TL,Theodoraki MN

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Thu Nov 16 00:00:00 UTC 2023

Blast-Derived Small Extracellular Vesicles in the Plasma of Patients with Acute Myeloid Leukemia Predict Responses to Chemotherapy.

Biomedicines

Boyiadzis M,Hong CS,Yerneni S,Im A,Diergaarde B,Whiteside TL

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Thu Dec 07 00:00:00 UTC 2023

Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics.

Frontiers in aging neuroscience

Cazzaro S,Fang C,Khan H,Witas R,Kee TR,Woo JA,Kang DE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue Aug 15 00:00:00 UTC 2023

Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics.

Frontiers in aging neuroscience

Cazzaro S,Fang C,Khan H,Witas R,Kee TR,Woo JA,Kang DE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue Aug 15 00:00:00 UTC 2023

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Mar 07 00:00:00 UTC 2022

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Mar 07 00:00:00 UTC 2022

Determining extracellular vesicles properties and miRNA cargo variability in bovine milk from healthy cows and cows undergoing subclinical mastitis.

BMC genomics

Saenz-de-Juano MD,Silvestrelli G,Bauersachs S,Ulbrich SE

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Mar 07 00:00:00 UTC 2022

Characteristics of Exosomes and the Vascular Landscape Regulate Exosome Sequestration by Peripheral Tissues and Brain.

International journal of molecular sciences

Banks WA,Sharma P,Hansen KM,Ludwig N,Whiteside TL

PA5-31260 was used in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to explore whether exosome tissue sequestration is determined by the exosomes or the tissues using ten radiolabeled exosomes from human or murine, cancerous or noncancerous cell lines.

Wed Oct 19 00:00:00 UTC 2022

Characteristics of Exosomes and the Vascular Landscape Regulate Exosome Sequestration by Peripheral Tissues and Brain.

International journal of molecular sciences

Banks WA,Sharma P,Hansen KM,Ludwig N,Whiteside TL

Wed Oct 19 00:00:00 UTC 2022

Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression.

Journal of extracellular vesicles

Pietrowska M,Zebrowska A,Gawin M,Marczak L,Sharma P,Mondal S,Mika J,Polańska J,Ferrone S,Kirkwood JM,Widlak P,Whiteside TL

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Feb 01 00:00:00 UTC 2021

Proteomic profile of melanoma cell-derived small extracellular vesicles in patients' plasma: a potential correlate of melanoma progression.

Journal of extracellular vesicles

Pietrowska M,Zebrowska A,Gawin M,Marczak L,Sharma P,Mondal S,Mika J,Polańska J,Ferrone S,Kirkwood JM,Widlak P,Whiteside TL

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Mon Feb 01 00:00:00 UTC 2021

Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.

International journal of molecular sciences

Almiñana C,Rudolf Vegas A,Tekin M,Hassan M,Uzbekov R,Fröhlich T,Bollwein H,Bauersachs S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.

International journal of molecular sciences

Almiñana C,Rudolf Vegas A,Tekin M,Hassan M,Uzbekov R,Fröhlich T,Bollwein H,Bauersachs S

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Small Extracellular Vesicles in Pre-Therapy Plasma Predict Clinical Outcome in Non-Small-Cell Lung Cancer Patients.

Cancers

Vetsika EK,Sharma P,Samaras I,Markou A,Georgoulias V,Whiteside TL,Kotsakis A

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Apr 23 00:00:00 UTC 2021

Small Extracellular Vesicles in Pre-Therapy Plasma Predict Clinical Outcome in Non-Small-Cell Lung Cancer Patients.

Cancers

Vetsika EK,Sharma P,Samaras I,Markou A,Georgoulias V,Whiteside TL,Kotsakis A

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Fri Apr 23 00:00:00 UTC 2021

DPP4⁺ exosomes in AML patients' plasma suppress proliferation of hematopoietic progenitor cells.

Leukemia

Namburi S,Broxmeyer HE,Hong CS,Whiteside TL,Boyiadzis M

PA5-31260 was used in Western Blotting to investigate the role of dipeptidylpeptidase4 (DPP4/CD26) carried by exosomes from acute myeloid leukemia (AML) plasma in suppressing hematopoiesis.

Thu Jul 01 00:00:00 UTC 2021

JC Virus infected choroid plexus epithelial cells produce extracellular vesicles that infect glial cells independently of the virus attachment receptor.

PLoS pathogens

O'Hara BA,Morris-Love J,Gee GV,Haley SA,Atwood WJ

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Sun Mar 01 00:00:00 UTC 2020

Induction of a proliferative response in the zebrafish retina by injection of extracellular vesicles.

Experimental eye research

Didiano D,Abner JJ,Hinger SA,Flickinger Z,Kent M,Clement MA,Balaiya S,Liu Q,Dai X,Levine EM,Patton JG

PA5-31260 was used in Western Blot to identify key factors that might target EVs to MG and initiate retina regeneration.

Sun Nov 01 00:00:00 UTC 2020

The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.

International journal of molecular sciences

Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue May 26 00:00:00 UTC 2020

The Potential of CD16 on Plasma-Derived Exosomes as a Liquid Biomarker in Head and Neck Cancer.

International journal of molecular sciences

Hofmann L,Ludwig S,Schuler PJ,Hoffmann TK,Brunner C,Theodoraki MN

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Tue May 26 00:00:00 UTC 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed Jul 29 00:00:00 UTC 2020

Circulating Exosomes Inhibit B Cell Proliferation and Activity.

Cancers

Schroeder JC,Puntigam L,Hofmann L,Jeske SS,Beccard IJ,Doescher J,Laban S,Hoffmann TK,Brunner C,Theodoraki MN,Schuler PJ

Published figure using TSG101 polyclonal antibody (Product # PA5-31260) in Western Blot

Wed Jul 29 00:00:00 UTC 2020

Enhanced generation of intraluminal vesicles in neuronal late endosomes in the brain of a Down syndrome mouse model with endosomal dysfunction.

Developmental neurobiology

D'Acunzo P,Hargash T,Pawlik M,Goulbourne CN,Pérez-González R,Levy E

PA5-31260 was used in Western Blotting to ivestigate the regulation of exosome secretion in Down syndrome and Alzheimer's postmortem brains in both human and mouse models.

Mon Jul 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

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Gene Aliases: AI255943; CC2; Rw; TSG10; TSG101; VPS23

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UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187

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Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088

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Function(s)

DNA binding transcription corepressor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity ubiquitin protein ligase binding protein homodimerization activity ubiquitin binding virion binding calcium-dependent protein binding ubiquitin-protein ligase

Process(es)

regulation of cell growth protein monoubiquitination autophagy cell cycle arrest negative regulation of cell proliferation endosome to lysosome transport protein transport endosomal transport viral life cycle keratinocyte differentiation multivesicular body assembly viral budding via host ESCRT complex ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway regulation of MAP kinase activity negative regulation of transcription, DNA-templated viral budding positive regulation of viral process cell division intracellular transport of virus autophagosome maturation regulation of viral release from host cell positive regulation of viral release from host cell positive regulation of exosomal secretion regulation of extracellular exosome assembly regulation of viral budding via host ESCRT complex positive regulation of viral budding via host ESCRT complex positive regulation of ubiquitin-dependent endocytosis cellular protein modification process cell differentiation regulation of growth regulation of nucleic acid-templated transcription transport cell cycle viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TSG101 Polyclonal Antibody

TSG101 Antibody (PA5-31260) in ICC/IF

Product Details

PA5-31260

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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