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Invitrogen
TSG101 Polyclonal Antibody
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FIGURE: 1 / 31
TSG101 Antibody (PA5-31260) in ICC/IF
Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with TSG101 Polyclonal Antibody (Product # PA5-31260, 1:100( in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:200... View More
Product Details
PA5-31260
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine,
C. elegans,
Horse,
Human,
Mouse,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TSG101. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.42 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, K562, THP-1, HL-60, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116, U87-MG, SK-N-SH, IMR32, SK-N-AS.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein
Gene Aliases: AI255943; CC2; Rw; TSG10; TSG101; VPS23
UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187
Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088
regulation of cell growth
protein monoubiquitination
autophagy
cell cycle arrest
negative regulation of cell proliferation
endosome to lysosome transport
protein transport
endosomal transport
viral life cycle
keratinocyte differentiation
multivesicular body assembly
viral budding via host ESCRT complex
ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
regulation of MAP kinase activity
negative regulation of transcription, DNA-templated
viral budding
positive regulation of viral process
cell division
intracellular transport of virus
autophagosome maturation
regulation of viral release from host cell
positive regulation of viral release from host cell
positive regulation of exosomal secretion
regulation of extracellular exosome assembly
regulation of viral budding via host ESCRT complex
positive regulation of viral budding via host ESCRT complex
positive regulation of ubiquitin-dependent endocytosis
cellular protein modification process
cell differentiation
regulation of growth
regulation of nucleic acid-templated transcription
transport
cell cycle
viral process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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