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Invitrogen
TSH Receptor Monoclonal Antibody (49)
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FIGURE: 1 / 3
TSH Receptor Antibody (MA3-218) in ICC/IF
Immunofluorescence analysis of Thyrotropin Receptor was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TSH Receptor (49) Mouse Monoclonal Antibody (Product # MA3-218) at 1:250 dilution in 0.1% BSA and incu... View More
Product Details
MA3-218
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
49
Immunogen
Purified extracellular domain of recombinant human thyrotropin receptor.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-218 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR.
MA3-218 has been successfully used in Western blot, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSHR respectively, in transfected Sf9 cell extract. By ELISA MA3-218 has been used in TSH competitive inhibition studies.
The MA3-218 antigen is purified extracellular domain of recombinant human thyrotropin receptor. This antibody recognizes an epitope between amino acid residues 277-296 of human TSHR.
Target Information
TSH Receptor gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: G-protein coupled receptor; TSHR; MGC141920; seven transmembrane helix receptor; Thyroid-stimulating hormone receptor; Thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH receptor; TSH-R; TSHR; seven transmembrane domain G protein-coupled receptor; unnamed protein product
Gene Aliases: CHNG1; hTSHR-I; LGR3; TSHR
UniProt ID: (Pig) Q8SPP9, (Human) P16473
Entrez Gene ID: (Pig) 397560, (Human) 7253
cell surface receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
thyroid-stimulating hormone signaling pathway
cellular response to glycoprotein
cellular response to thyrotropin-releasing hormone
signal transduction
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
cell-cell signaling
positive regulation of cell proliferation
response to hormone
hormone-mediated signaling pathway
positive regulation of cold-induced thermogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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