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Invitrogen
TSLP Polyclonal Antibody
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FIGURE: 1 / 5
TSLP Antibody (PA5-20321) in IHC (P)
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using TSLP Polyclonal Antibody (Product # PA5-20321) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. ... View More
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.jpg?time=20220826?time=20220826 "TSLP Antibody (PA5-20321) in IHC")
Product Details
PA5-20321
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 20 amino acid peptide from near the center of mouse TSLP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is A-20 cell lysate.
PA5-20321 can be used with blocking peptide PEP-0441.
Target Information
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Thymic stroma-derived lymphopoietin; Thymic stromal lymphopoietin
Gene Aliases: Tslp
UniProt ID: (Mouse) Q9JIE6
Entrez Gene ID: (Rat) 688621, (Mouse) 53603
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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