|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid synthetic peptide near the amino terminus of human TTYH2|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2.5 ug/ml|
|Immunofluorescence (IF)||2.5 ug/mL|
|Immunohistochemistry (IHC)||2.5 ug/mL|
|Western Blot (WB)||1 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is human kidney tissue lysate.
PA5-34395 can be used with blocking peptide PEP-1437.
TTYH2 is a member of the tweety family of proteins, a family of chloride anion channels containing five transmembrane regions. TTYH2 is a Ca2+-activated large conductance chloride (Cl-) channel and may play a role in kidney tumorigenesis. It is also upregulated in colon carcinoma and is thought to play a role in cell proliferation and call aggregation, suggesting that TTYH2 may play a role in regulating both proliferation and metastatic potentials of colorectal cancer. The ubiquitin-protein ligase Nedd4-2 binds to and ubiquitinates TTYH2, thereby regulating its cell surface expression and total cellular levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.