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|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 189 and 377 of Human TUFM|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 40% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-27512 targets TUFM in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27512 immunogen is recombinant fragment corresponding to a region within amino acids 189 and 377 of Human TUFM.
This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses.
PA5-27512 was used in western blot to examine the activated signaling pathways and pathogen resistance of rice plants expressing Arabidopsis EFR or the chimeric receptor EFR::XA21.
|Schwessinger B,Bahar O,Thomas N,Thomas N,Holton N,Nekrasov V,Ruan D,Canlas PE,Daudi A,Petzold CJ,Singan VR,Kuo R,Chovatia M,Daum C,Heazlewood JL,Zipfel C,Ronald PC||PLoS pathogens (11:null)||2015|
COXPD4; EF-Tu; EF-TuMT; EFTU; elongation factor Tu; mitochondrial
COXPD4; EF-TuMT; EFTU; P43; TUFM