Proteintech

TULP3 Polyclonal Antibody

View all (20) TULP3 antibodies

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Questions & Answers

Tech Support

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TULP3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 13637-1-AP (TULP3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TULP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TULP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TULP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TULP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13637-1-AP (TULP3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TULP3 Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13637-1-AP (TULP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

TULP3 Antibody in Immunoprecipitation (IP) — IP result of anti-TULP3 (IP: 13637-1-AP, 4 µg; Detection: 13637-1-AP 1:800) with SH-SY5Y cells lysate 1600 µg.

Product Details

13637-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TULP3 Fusion Protein Ag4570 (96-442 aa encoded by BC032587)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: DEVHAPSVS SSVVEEDAEN TVDTASKPGL QERLQKHDIS ESVNFDEETD GISQSACLER PNSASSQNST DTGTSGSATA AQPADNLLGD IDYLEDFVYS PAPQGVTVRC RIIRDKRGMD RGLFPTYYMY LEKEENQKIF LLAARKRKKS KTANYLISID PVDLSREGES YVGKLRSNLM GTKFTVYDRG ICPMKGRGLV GAAHTRQELA AISYETNVLG FKGPRKMSVI IPGMTLNHKQ IPYQPQNNHD SLLSRWQNRT MENLVELHNK APVWNSDTQS YVLNFRGRVT QASVKNFQIV HKNDPDYIVM QFGRVADDVF TLDYNYPLCA VQAFGIGLSS FDSKLACE (96-442 aa encoded by BC032587 )

Target Information

This gene encodes a member of the tubby-like protein (TULP) family. Members of this family have been identified in plants, vertebrates, and invertebrates, and they share a conserved C-terminal region of approximately 200 amino acid residues. The human and mouse TULP3 proteins share 69% amino acid sequence identity, with higher identity at the N and C termini than in the central region.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti.

Frontiers in neuroscience

Lindner LA,Derstroff D,Oliver D,Reimann K

Sun May 07 00:00:00 EDT 2023

Identification of a heterogeneous and dynamic ciliome during embryonic development and cell differentiation.

Development (Cambridge, England)

Elliott KH,Balchand SK,Bonatto Paese CL,Chang CF,Yang Y,Brown KM,Rasicci DT,He H,Thorner K,Chaturvedi P,Murray SA,Chen J,Porollo A,Peterson KA,Brugmann SA

Sat Apr 15 00:00:00 EDT 2023

Multiple ciliary localization signals control INPP5E ciliary targeting.

eLife

Cilleros-Rodriguez D,Martin-Morales R,Barbeito P,Deb Roy A,Loukil A,Sierra-Rodero B,Herranz G,Pampliega O,Redrejo-Rodriguez M,Goetz SC,Izquierdo M,Inoue T,Garcia-Gonzalo FR

Mon Sep 05 00:00:00 EDT 2022

Multiple ciliary localization signals control INPP5E ciliary targeting.

eLife

Cilleros-Rodriguez D,Martin-Morales R,Barbeito P,Deb Roy A,Loukil A,Sierra-Rodero B,Herranz G,Pampliega O,Redrejo-Rodriguez M,Goetz SC,Izquierdo M,Inoue T,Garcia-Gonzalo FR

Mon Sep 05 00:00:00 EDT 2022

The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling.

Cell reports

Schembs L,Willems A,Hasenpusch-Theil K,Cooper JD,Whiting K,Burr K,Bøstrand SMK,Selvaraj BT,Chandran S,Theil T

Tue May 17 00:00:00 EDT 2022

MicroRNA-506 has a suppressive effect on the tumorigenesis of nonsmall-cell lung cancer by regulating tubby-like protein 3.

Bioengineered

Li ZH,Zhou JH,Chen SN,Pan L,Feng Y,Luo MQ,Li RX,Sun GL

Wed Dec 01 00:00:00 EST 2021

MicroRNA-506 has a suppressive effect on the tumorigenesis of nonsmall-cell lung cancer by regulating tubby-like protein 3.

Bioengineered

Li ZH,Zhou JH,Chen SN,Pan L,Feng Y,Luo MQ,Li RX,Sun GL

Wed Dec 01 00:00:00 EST 2021

MicroRNA-506 has a suppressive effect on the tumorigenesis of nonsmall-cell lung cancer by regulating tubby-like protein 3.

Bioengineered

Li ZH,Zhou JH,Chen SN,Pan L,Feng Y,Luo MQ,Li RX,Sun GL

Wed Dec 01 00:00:00 EST 2021

MicroRNA-506 has a suppressive effect on the tumorigenesis of nonsmall-cell lung cancer by regulating tubby-like protein 3.

Bioengineered

Li ZH,Zhou JH,Chen SN,Pan L,Feng Y,Luo MQ,Li RX,Sun GL

Wed Dec 01 00:00:00 EST 2021

ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Biology open

Fujisawa S,Qiu H,Nozaki S,Chiba S,Katoh Y,Nakayama K

Wed Sep 15 00:00:00 EDT 2021

Comparison of Ciliary Targeting of Two Rhodopsin-Like GPCRs: Role of C-Terminal Localization Sequences in Relation to Cilium Type.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chadha A,Paniagua AE,Williams DS

Wed Sep 08 00:00:00 EDT 2021

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Molecules and cells

Hong JJ,Kim KE,Park SY,Bok J,Seo JT,Moon SJ

Tue Aug 31 00:00:00 EDT 2021

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Molecules and cells

Hong JJ,Kim KE,Park SY,Bok J,Seo JT,Moon SJ

Tue Aug 31 00:00:00 EDT 2021

Deletion of the phosphatase INPP5E in the murine retina impairs photoreceptor axoneme formation and prevents disc morphogenesis.

The Journal of biological chemistry

Sharif AS,Gerstner CD,Cady MA,Arshavsky VY,Mitchell C,Ying G,Frederick JM,Baehr W

Mon Aug 23 00:00:00 EDT 2021

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Science advances

Chen C,Xu Q,Zhang Y,Davies BA,Huang Y,Katzmann DJ,Harris PC,Hu J,Ling K

Tue Jun 01 00:00:00 EDT 2021

RABL2 positively controls localization of GPCRs in mammalian primary cilia.

Journal of cell science

Dateyama I,Sugihara Y,Chiba S,Ota R,Nakagawa R,Kobayashi T,Itoh H

Wed Jan 16 00:00:00 EST 2019

Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.

PLoS genetics

Hong CJ,Hamilton BA

Sat Oct 01 00:00:00 EDT 2016

Zfp423 Regulates Sonic Hedgehog Signaling via Primary Cilium Function.

PLoS genetics

Hong CJ,Hamilton BA

Sat Oct 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Tubby-like protein 3; Tubby-related protein 3

Gene Aliases: 2310022L06Rik; AI316887; TUBL3; TULP3

UniProt ID: (Human) O75386, (Mouse) O88413

Entrez Gene ID: (Human) 7289, (Mouse) 22158, (Rat) 688749

neural tube formation neural tube closure multicellular organism development brain development regulation of G-protein coupled receptor protein signaling pathway regulation of smoothened signaling pathway anterior/posterior pattern specification dorsal/ventral neural tube patterning negative regulation of smoothened signaling pathway involved in ventral spinal cord patterning neural tube development central nervous system neuron differentiation embryonic camera-type eye development embryonic digit morphogenesis negative regulation of smoothened signaling pathway embryonic neurocranium morphogenesis limb development bone development bronchus morphogenesis smoothened signaling pathway involved in dorsal/ventral neural tube patterning ganglion development receptor localization to nonmotile primary cilium negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning protein localization to photoreceptor outer segment regulation of transcription, DNA-templated G-protein coupled receptor signaling pathway

Process(es)

protein binding phosphatidylinositol-4,5-bisphosphate binding enzyme binding protein complex binding phosphatidylinositol binding