Western blot analysis of TdT using A) 30 µg 293T whole cell extract and B) 30 µg whole cell extract of human DNTT-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TdT polyclonal antibody (Product # PA5-28880) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 296 and 509 of Human TdT|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28880 targets TdT in WB applications and shows reactivity with Human samples.
The PA5-28880 immunogen is recombinant fragment corresponding to a region within amino acids 296 and 509 of Human TdT.
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
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