|Tested species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an internal portion of Escherichia coli TetR protein (between residues 25-75)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is not reactive to human or other mammalian proteins.
The 23 kDa Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or represor domains are fused to TetR to turn it into an efficient regulator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.