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Agrisera
Tic40/Inner envelope membrane translocon complex protein (chloroplast) Polyclonal Antibody
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Tic40/Inner envelope membrane translocon complex protein (chloroplast) Antibody (AS10-709) in WB
10-15 µg of chlorophyll from isolated total plant material (Arabidopsis thaliana), chloroplasts and thylakoids extracted with a buffer containing (25 m M Tricine-Na OH, p H 7.8,330 m M sorbitol, 1 m M EDTA, 10 m M KCl, 0.15% [w/v] bovine serum albumin, 4 m M sodium ascorbate, and 7 m M L-Cys) were separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 10% milk for... View More
Product Details
AS10-709
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from available plant sequences of Tic40.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidosps thaliana, Catharantus roseus, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Solanum lycopersicum, Triticum aestivum, Zea mays, Vitis vinifera
Target Information
Tic40 encodes a nucleus-derived chloroplast preprotein import factor that is targeted to the chloroplast envelope and resides as a single-pass, inner envelope membrane-anchored component of the TIC translocon, with a short N-terminal membrane anchor and a large hydrophilic C-terminal domain exposed to the stroma. Structurally, the stromal domain contains a tetratricopeptide repeat (TPR) region followed by a C-terminal module with similarity to the Sti1/Hop and Hip classes of co-chaperones, consistent with a role in organizing chaperone interactions at the import site. Functionally, Tic40 acts during the translocation step across the inner envelope rather than initial precursor binding, physically and functionally coupling core TIC components such as Tic110 with stromal chaperone machinery including Hsp93 (Hsp100 family) to facilitate efficient pulling and/or handoff of incoming preproteins into the stroma; loss of Tic40 reduces import efficiency and impairs chloroplast biogenesis, yielding chlorotic, slow-growing plants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATTIC40; MTG13.14; MTG13_14; PDE120; pigment defective embryo; TIC40
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