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Bethyl Laboratories
Topo II Alpha Polyclonal Antibody
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FIGURE: 1 / 3
Topo II Alpha Antibody (A300-054A) in IHC (P)
Detection of human Topo II Alpha by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II alpha (Product # A300-054A) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
Product Details
A300-054A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1500 and the C-terminus (residue 1531) of Human Topoisomerase (DNA) II Alpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II (EC 5.99.1.3); DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; TOP2a/90; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha
Gene Aliases: TOP2; TOP2A; TOP2alpha; TOPIIA; TP2A
UniProt ID: (Human) P11388
Entrez Gene ID: (Human) 7153
nucleotide binding
magnesium ion binding
DNA binding
chromatin binding
RNA binding
DNA topoisomerase activity
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein binding
ATP binding
DNA-dependent ATPase activity
DNA binding, bending
isomerase activity
protein homodimerization activity
ubiquitin binding
metal ion binding
protein heterodimerization activity
resolution of meiotic recombination intermediates
sister chromatid segregation
hematopoietic progenitor cell differentiation
DNA metabolic process
DNA topological change
chromatin organization
cellular response to DNA damage stimulus
chromosome segregation
female meiotic division
chromosome condensation
apoptotic chromosome condensation
embryonic cleavage
regulation of circadian rhythm
positive regulation of apoptotic process
positive regulation of single stranded viral RNA replication via double stranded DNA intermediate
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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